NEDD4 2 (NEDD4L) Human Gene Knockout Kit (CRISPR)

CAT#: KN217897

NEDD4L - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: NEDD4 2
• Locus ID: 23327
• Components:

  KN217897G1, NEDD4 2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTATGGACTTTCCGAAGACG

  KN217897G2, NEDD4 2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGCGGGATAGGAACTCCCCG

  KN217897D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GCCCCGGGCC GGGGTGGGCG TGGGGCTGAA CTCCGCCACC CCCGCAGCCC GCGGCCCGCT CGCCAGCCCG GCCTGTGATT GGCTTGGGGG GCGCGTGTGC CCGCCCCGGC GAACGGGGAG GGTACCCTCG CGGCGCCGGT GGGAGGAGGC GGAGGAGGGG GCGGAGAGAG CGGGGGCCCG GACGGCGCTA GCGAGGAGGC GGGCGAGCCG GGTCCCCGGG AGCGCAGAGG AGGCTCGGAG GGGGGCGGAG AGCGGCGGGG CGGGAGGGAG GCGCGGGTCG CGGGGAGGGA AAGCCCGGCC GGGTCTGCGC CGCCGCCGCG CGCAGTGAGA GGCGCCGGGG CTGCCGCCCG GTGCTCGGCG CGCTCTCGGG AGCCGCCCGC CCGCTGGTCC CGCAGCCTTC CGGGAGGAAG CGGTGCCGGC AGCGTCCAGG GCGCGCTCTC GGGCACCCTC ACCTGCCGGC GCCCGGCCGC TTACCCGGCA GGGCGTGCGC AGGGTAGGGT GCGGGACCGG GGGGACCTGG AGGCAGAGGG GAGAACCGGC CGTCCGCGCC GCAGCACAGC CGCTGGGAGC GCCTCAGACC CCGCGCGGGG CGCCGGCTCC GAAAGGGGCC GTCCCCGGGG TGCTCGTGCC CAGCGTCTGC AGGGGAGCCC CAAAGCGGGA GCGCCCCGGA GCGGGATCCA GGGGAGCTTG GGTCCTTCCG CACCCCCCAC CCTCCGCAAG CCGAGCTCAT TGTTTGTAAA TAAAGCGGCG CGACGCCCTT GGAGCTGGGG GTTCACTCCG CAGCTCCTCG CTTTCGGGAG GAGAGGGAGG GGGCATGCGT GCCTCTCTTC CCTCTTCCTC TCCAAGCAGC GTCTCCCGGG GCTGTTGGGC AACTTTCCGC CTCTCGCCCC TGCAGCCCGG GAGTCCATGA TGCATGTCAC GTCTGGGTCG CGCGGCTGGA GACCCGGGAC TGCGGAGAAG CCGGATCGTT TCCCCCTGGA ACTTTCTCTC TCATGATTGT GTGGATCAGC AGGCGCTCAG AAGCCTGTGC GTGCTTATCC CGGCGGGAAT GGGGGACACG CTGCGTGTGT CCCTCCCCTC GTTTTGCAAA GTCTACGGCC AGGATTGTGG ATTTCGGAGC CTCGGTGGGG GAGGTGTTCA AGGCTGGTGG CAGGTGCTCC TGGAAACTTG GGGAGGAAGC TTGCAGCCCT GCCATTTTTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001144964, NM_001144965, NM_001144966, NM_001144967, NM_001144968, NM_001144969, NM_001144970, NM_001144971, NM_001243960, NM_015277
• UniProt ID: Q96PU5
• Synonyms: hNEDD4-2; NEDD4-2; NEDD4.2; RSP5
• Summary: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]