TMEM97 Human Gene Knockout Kit (CRISPR)

CAT#: KN216927

TMEM97 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: TMEM97
• Locus ID: 27346
• Components:

  KN216927G1, TMEM97 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TCGACTGGGTAGAGCTCGCG

  KN216927G2, TMEM97 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCTGTTCATGGACCTGCAGG

  KN216927D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TTTTGTAGAG ATAATGTCTT ACTAAGTTAC CCAGGCTGGT CTTCTGGGCT CAAGCCATCC TCCTGCCTCG GGCTCCCAAA GTGCAGACAT TACAGGCATG ACCCACCGTG CACAGCGTCA GCTTTATTTT TTTAATGCTG ACTTACTGCA GAAGGCCCAA GGGCCAGTGC AGTTTTCATA CTGCTGTTGT AGTGGCGAAC GGAGCCACGA GGGAAGGGAG CGCCGCCGGC AGCTGCCGGG ACACCAGGAT ACCACGCGGC CAGCACCGAA ACGCTAGGCT CGCGCTCACA ACCGCAATCT ACACTGAGTA GCCGCGCGCC GAACGGCGTG ACTAAGCCCC GCCCACACGG CGCTCCTGGC GGCCAATGGA AATGGCTTAC GAGACGCCTT CTCCACGCGC TATACTTATA CGGACCAATG GGTGCGCCCT GTGGGGAGTG CCGCCCCCTG TCCACGAGCC TCCTCTTCTC GCCAGCCAAT GGAAGCGGTC TGCCTAGCCC TCGCGGGGGC AAGGCGGCGA CCGAGGCGCG TGGGTCTGGG AAGGCGCGCG GATTTGGCCC CTCTTCTCAC ATCAGCGGGT CCAGGCCCAA CCGACAGACT TCTTATCCCG GCCCGCTGAG GCTCTCCCGG CGCTGCGTCC ACAGGGCCTT CACCTCCTCC GCGCTCGCGC ACTCTGGGTT CCAGTTGCCT CTCTCGGGTC CTGCCCATGC CTCCCCCGCT CCTAACCCAA CTTTAGTTCG GTGTTTTCCT CGGGCTTTGT CTCTATCCCA GCCCCTTCTC CCAAGCGGCG GGGGCGAACG CTTTGTTTCC TTTAAAGTCA CTTCTTCCCT GGGTTTAAGG TTTCCCGCGA TTCCACCTCT GGCCGGTTGT CAGTGTCTGA GGGAAGTAGT CTGGATGAAG TGGTGTCCAA AGTCACATGT TAGGTTTGCT TTAAGCTTCT GGTTTTTTGT TTGTTTGTTT GGAAGGGCTT TAAAATACCA GTCTGATCCC AAAGAAAAAA TCCGAGTAGA AATAGACCTG TCTGAAGGTT TAGCACCTTG GTTGGTGCAG CTGGGGCGTT CACAGATCGT CACTGCTTCG AGATCAGGTT CCAGCAACTT CCAGAGCTCA GGAGTAAATT GCCATCTTTT CAGATTGGCT CTTCAATATT GGGTTCGAGT AACAATGATC TCCTGAGTAA AAATAAAGGT GAAAATCCCG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_014573
• UniProt ID: Q5BJF2
• Synonyms: MAC30
• Summary: TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels (Bartz et al., 2009 [PubMed 19583955]).[supplied by OMIM, Aug 2009]