Parkin (PARK2) Human Gene Knockout Kit (CRISPR)

CAT#: KN216186LP

PARK2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: Parkin
• Locus ID: 5071
• Components:

  KN216186G1, Parkin gRNA vector 1 in pCas-Guide CRISPR vector

  KN216186G2, Parkin gRNA vector 2 in pCas-Guide CRISPR vector

  KN216186LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_004562, NM_013987, NM_013988
• UniProt ID: O60260
• Synonyms: AR-JP; LPRS2; PDJ; PRKN
• Summary: The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008]