ITIH5 Human Gene Knockout Kit (CRISPR)

CAT#: KN214478

ITIH5 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: ITIH5
• Locus ID: 80760
• Components:

  KN214478G1, ITIH5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCAACAGTCTGACTTGCCTC

  KN214478G2, ITIH5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GAGGCTGGTAGGTATATTCA

  KN214478D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CCACTGCCCT CCAACCTGAG CAACACAGCG AGGCCATGTC TAAAAAAAGA CGATTTCATC ACTTTCCCGC ATCCCTCCCC GGCATGTGGA AGCATATAAG AAATCATCCA GAAAAGACAC CATGAAGAAA ATTCAAGATA AGTTCAGTTT TGCTTCCAGA GTTTCCTGGA GTTTTAAGTT GAATAGTGAG CCCACTGGAA TTAAGACTGA ATACCTTTAC TTACTGTAAT AGACACAGTA GCAAAGAGTT TAACTTGAGG CCCCAAATAA AATGAAACAG AATACAAATA CTCTATCTTT TTGTTCTTGT CATTTCATCC ATCTCCTCTT ACAGATAGAA GAATGCTCAG GCGATGAGAT AGTGTGACAT TATGGGTTAA GAGTCAACCA GATCTGACAG GGATGAATTC TAATTCTGGC CTCGAAACAA ACTCCCTGAT AAAAAGTCAA AACTGCTTTT CAGGATAGAT ACAGCTTTTG GTGAATCTGA ATAGATTTTA ACCCCCTTGT ATAACTCATG TGAGAATGAC CTCACAATCA TATTTCATGT CTCTTCTCTT TTAAAAATCA CCTTTTTAAC AGTCTCTTCT CTTTTCTAGG TTCCCTCATT CTTCTATTTA TTTTGAACCA GGTATCCACT TTGCTTGCAG ATCCTCCAAA ACATATAAAG TTGCTTGTAG AAAAATTCTG AGATGAAACA GCATAGTTGA GTCTTTTATA AGGCTTTCTT TAAGTGGATT TTTTTTTTCT TTTTTCTGGA AGGAGACCTG ATTATTTCAG TAGAACTAAA AACACAAAGG TCTTTGCAGG TTTATTTTTT TTAAAGCCTA AATATAACTT TTATTTATTT TTATGACATC CTTACTCAGG AAATGAGAGC TTTGTTGTGA AGTAAACAAC TAAAGCAACC AAAGAATTGA CATTTGTAGA CTAAAGCATC ACGTGATGAA AGTTGGATAG GCTCTCAATG CTGCTGGAGG CTAAGAGATT GGGACCGGGG CAAATGTTCA GAAGCTCTAA GAGCTTATTT GAAACTACCT CAGTTCTTCC TTACCACTGT GCATGGAGTG AAATATCAAA ATGTAGAGAC ATGCCTTTAG ACTCTGGTGA GGCATTTATC CTAGCCCTCC CTGCACCTGG CTGGTGTTTG AGTGGATTCA GATTTCTTGT CATTACCAAT ATCACAATCA TGACTATCTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001001851, NM_030569, NM_032817
• UniProt ID: Q86UX2
• Synonyms: ITI-HC5; PP14776
• Summary: This gene encodes a heavy chain component of one of the inter-alpha-trypsin inhibitor (ITI) family members. ITI proteins are involved in extracellular matrix stabilization and in the prevention of tumor metastasis. They are also structurally related plasma serine protease inhibitors and are composed of a light chain and varying numbers of heavy chains. This family member is thought to function as a tumor suppressor in breast and thyroid cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]