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ITIH5 (NM_030569) Human Tagged ORF Clone

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SKU
RC214536
ITIH5 (Myc-DDK-tagged)-Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $875.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ITIH5
Synonyms ITI-HC5; PP14776
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214536 representing NM_030569
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCTGCTGCTGGGGCTGTGCCTGGGGCTGTCCCTGTGTGTGGGGTCGCAGGAAGAGGCGCAGAGCT
GGGGCCACTCTTCGGAGCAGGATGGACTCAGGGTCCCGAGGCAAGTCAGACTGTTGCAGAGGCTGAAAAC
CAAACCTTTGATGACAGAATTCTCAGTGAAGTCTACCATCATTTCCCGTTATGCCTTCACTACGGTTTCC
TGCAGAATGCTGAACAGAGCTTCTGAAGACCAGGACATTGAGTTCCAGATGCAGATTCCAGCTGCAGCTT
TCATCACCAACTTCACTATGCTTATTGGAGACAAGGTGTATCAGGGCGAAATTACAGAGAGAGAAAAGAA
GAGTGGTGATAGGGTAAAAGAGAAAAGGAATAAAACCACAGAAGAAAATGGAGAGAAGGGGACTGAAATA
TTCAGAGCTTCTGCAGTGATTCCCAGCAAGGACAAAGCCGCCTTTTTCCTGAGTTATGAGGAGCTTCTGC
AGAGGCGCCTGGGCAAGTACGAGCACAGCATCAGCGTGCGGCCCCAGCAGCTGTCCGGGAGGCTGAGCGT
GGACGTGAATATCCTGGAGAGCGCGGGCATCGCATCCCTGGAGGTGCTGCCGCTTCACAACAGCAGGCAG
AGGGGCAGTGGGCGCGGGGAAGATGATTCTGGGCCTCCCCCATCTACTGTCATTAACCAAAATGAAACAT
TTGCCAACATAATTTTTAAACCTACTGTAGTACAACAAGCCAGGATTGCCCAGAATGGAATTTTGGGAGA
CTTTATCATTAGATATGACGTCAATAGAGAACAGAGCATTGGGGACATCCAGGTTCTAAATGGCTATTTT
GTGCACTACTTTGCTCCTAAAGACCTTCCTCCTTTACCCAAGAATGTGGTATTCGTGCTTGACAGCAGTG
CTTCTATGGTGGGAACCAAACTCCGGCAGACCAAGGATGCCCTCTTCACAATTCTCCATGACCTCCGACC
CCAGGACCGTTTCAGTATCATTGGATTTTCCAACCGGATCAAAGTATGGAAGGACCACTTGATATCAGTC
ACTCCAGACAGCATCAGGGATGGGAAAGTGTACATTCACCATATGTCACCCACTGGAGGCACAGACATCA
ACGGGGCCCTGCAGAGGGCCATCAGGCTCCTCAACAAGTACGTGGCCCACAGTGGCATTGGAGACCGGAG
CGTGTCCCTCATCGTCTTCCTGACGGATGGGAAGCCCACGGTCGGGGAGACGCACACCCTCAAGATCCTC
AACAACACCCGAGAGGCCGCCCGAGGCCAAGTCTGCATCTTCACCATTGGCATCGGCAACGACGTGGACT
TCAGGCTGCTGGAGAAACTGTCGCTGGAGAACTGTGGCCTCACACGGCGCGTGCACGAGGAGGAGGACGC
AGGCTCGCAGCTCATCGGGTTCTACGATGAAATCAGGACCCCGCTCCTCTCTGACATCCGCATCGATTAT
CCCCCCAGCTCAGTGGTGCAGGCCACCAAGACCCTGTTCCCCAACTACTTCAACGGCTCGGAGATCATCA
TTGCGGGGAAGCTGGTGGACAGGAAGCTGGATCACCTGCACGTGGAGGTCACCGCCAGCAACAGTAAGAA
ATTCATCATCCTGAAGACAGATGTGCCTGTGCGGCCTCAGAAGGCAGGGAAAGATGTCACAGGAAGCCCC
AGGCCTGGAGGCGATGGAGAGGGGGACACCAACCACATCGAGCGTCTCTGGAGCTACCTCACCACAAAGG
AGCTGCTGAGCTCCTGGCTGCAAAGTGACGATGAACCGGAGAAGGAGCGGCTGCGGCAGCGGGCCCAGGC
CCTGGCTGTGAGCTACCGCTTCCTCACTCCCTTCACCTCCATGAAGCTGAGGGGGCCGGTCCCACGCATG
GATGGCCTGGAGGAGGCCCACGGCATGTCGGCTGCCATGGGACCCGAACCGGTGGTGCAGAGCGTGCGAG
GAGCTGGCACGCAGCCAGGACCTTTGCTCAAGAAGCCATACCAGCCAAGAATTAAAATCTCTAAAACATC
AGTGGATGGTGATCCCCACTTTGTTGTGGATTTCCCCCTGAGCAGACTCACCGTGTGCTTCAACATTGAT
GGGCAGCCCGGGGACATCCTCAGGCTGGTCTCTGATCACAGGGACTCTGGTGTCACAGTGAACGGAGAGT
TAATTGGGGCACCCGCCCCTCCAAATGGCCACAAGAAACAGCGCACTTACTTGCGCACTATCACCATCCT
CATCAACAAGCCAGAGAGATCTTATCTCGAGATCACACCGAGCAGAGTCATCTTGGATGGTGGGGACAGA
CTGGTGCTCCCCTGCAACCAGAGTGTGGTGGTGGGGAGCTGGGGGCTGGAGGTGTCCGTGTCTGCCAACG
CCAATGTCACCGTCACCATCCAGGGCTCCATAGCCTTTGTCATCCTCATCCACCTCTACAAAAAGCCGGC
GCCCTTCCAGCGACACCACCTGGGTTTCTACATTGCCAACAGCGAGGGCCTTTCCAGCAACTGCCACGGA
CTGCTGGGTCAGTTCCTGAATCAGGATGCCAGACTCACAGAAGACCCTGCAGGGCCCAGCCAGAACCTCA
CTCACCCTCTGCTCCTTCAGGTGGGAGAGGGGCCTGAGGCCGTCCTAACAGTGAAAGGCCACCAAGTCCC
AGTGGTCTGGAAGCAAAGGAAGATTTACAACGGGGAAGAGCAGATAGACTGCTGGTTTGCCAGGAACAAT
GCCGCCAAACTGATTGACGGGGAGTACAAGGATTACCTGGCATCCCATCCATTTGACACAGGGATGGACA
CTTGGCCGGGGAATGTCCAGGGAGCTCTGAAGCTGGCAGCCTTAAAGATGCAAGTGCATGAAGGACAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214536 representing NM_030569
Red=Cloning site Green=Tags(s)

MLLLLGLCLGLSLCVGSQEEAQSWGHSSEQDGLRVPRQVRLLQRLKTKPLMTEFSVKSTIISRYAFTTVS
CRMLNRASEDQDIEFQMQIPAAAFITNFTMLIGDKVYQGEITEREKKSGDRVKEKRNKTTEENGEKGTEI
FRASAVIPSKDKAAFFLSYEELLQRRLGKYEHSISVRPQQLSGRLSVDVNILESAGIASLEVLPLHNSRQ
RGSGRGEDDSGPPPSTVINQNETFANIIFKPTVVQQARIAQNGILGDFIIRYDVNREQSIGDIQVLNGYF
VHYFAPKDLPPLPKNVVFVLDSSASMVGTKLRQTKDALFTILHDLRPQDRFSIIGFSNRIKVWKDHLISV
TPDSIRDGKVYIHHMSPTGGTDINGALQRAIRLLNKYVAHSGIGDRSVSLIVFLTDGKPTVGETHTLKIL
NNTREAARGQVCIFTIGIGNDVDFRLLEKLSLENCGLTRRVHEEEDAGSQLIGFYDEIRTPLLSDIRIDY
PPSSVVQATKTLFPNYFNGSEIIIAGKLVDRKLDHLHVEVTASNSKKFIILKTDVPVRPQKAGKDVTGSP
RPGGDGEGDTNHIERLWSYLTTKELLSSWLQSDDEPEKERLRQRAQALAVSYRFLTPFTSMKLRGPVPRM
DGLEEAHGMSAAMGPEPVVQSVRGAGTQPGPLLKKPYQPRIKISKTSVDGDPHFVVDFPLSRLTVCFNID
GQPGDILRLVSDHRDSGVTVNGELIGAPAPPNGHKKQRTYLRTITILINKPERSYLEITPSRVILDGGDR
LVLPCNQSVVVGSWGLEVSVSANANVTVTIQGSIAFVILIHLYKKPAPFQRHHLGFYIANSEGLSSNCHG
LLGQFLNQDARLTEDPAGPSQNLTHPLLLQVGEGPEAVLTVKGHQVPVVWKQRKIYNGEEQIDCWFARNN
AAKLIDGEYKDYLASHPFDTGMDTWPGNVQGALKLAALKMQVHEGQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030569
ORF Size 2868 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030569.3, NP_085046.3
RefSeq Size 6331 bp
RefSeq ORF 2829 bp
Locus ID 80760
UniProt ID Q86UX2, C9J2H1
Cytogenetics 10p14
Domains VIT, VWA
MW 105.9 kDa
Summary This gene encodes a heavy chain component of one of the inter-alpha-trypsin inhibitor (ITI) family members. ITI proteins are involved in extracellular matrix stabilization and in the prevention of tumor metastasis. They are also structurally related plasma serine protease inhibitors and are composed of a light chain and varying numbers of heavy chains. This family member is thought to function as a tumor suppressor in breast and thyroid cancers. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2011
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"NM_030569" in other vectors (4)
SKU Description Size Price
RC214536L3 Lenti-ORF clone of ITIH5 (Myc-DDK-tagged)-Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1 10 ug
$1,175.00
RC214536L4 Lenti-ORF clone of ITIH5 (mGFP-tagged)-Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1 10 ug
$1,175.00
RG214536 ITIH5 (tGFP-tagged) - Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1 10 ug
$789.00 $1,075.00
SC315637 ITIH5 (untagged)-Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1 10 ug
$950.00

Plasmid Map for RC214536

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214536
Circular map for RC214536

file-alt Citations

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