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ITIH5 Human Gene Knockout Kit (CRISPR)

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SKU
KN214478
ITIH5 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Other products for "ITIH5 or NM_001001851"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol ITIH5
Locus ID 80760
Components

KN214478G1, ITIH5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCAACAGTCTGACTTGCCTC

KN214478G2, ITIH5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GAGGCTGGTAGGTATATTCA

KN214478D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

CCACTGCCCT CCAACCTGAG CAACACAGCG AGGCCATGTC TAAAAAAAGA CGATTTCATC ACTTTCCCGC ATCCCTCCCC GGCATGTGGA AGCATATAAG AAATCATCCA GAAAAGACAC CATGAAGAAA ATTCAAGATA AGTTCAGTTT TGCTTCCAGA GTTTCCTGGA GTTTTAAGTT GAATAGTGAG CCCACTGGAA TTAAGACTGA ATACCTTTAC TTACTGTAAT AGACACAGTA GCAAAGAGTT TAACTTGAGG CCCCAAATAA AATGAAACAG AATACAAATA CTCTATCTTT TTGTTCTTGT CATTTCATCC ATCTCCTCTT ACAGATAGAA GAATGCTCAG GCGATGAGAT AGTGTGACAT TATGGGTTAA GAGTCAACCA GATCTGACAG GGATGAATTC TAATTCTGGC CTCGAAACAA ACTCCCTGAT AAAAAGTCAA AACTGCTTTT CAGGATAGAT ACAGCTTTTG GTGAATCTGA ATAGATTTTA ACCCCCTTGT ATAACTCATG TGAGAATGAC CTCACAATCA TATTTCATGT CTCTTCTCTT TTAAAAATCA CCTTTTTAAC AGTCTCTTCT CTTTTCTAGG TTCCCTCATT CTTCTATTTA TTTTGAACCA GGTATCCACT TTGCTTGCAG ATCCTCCAAA ACATATAAAG TTGCTTGTAG AAAAATTCTG AGATGAAACA GCATAGTTGA GTCTTTTATA AGGCTTTCTT TAAGTGGATT TTTTTTTTCT TTTTTCTGGA AGGAGACCTG ATTATTTCAG TAGAACTAAA AACACAAAGG TCTTTGCAGG TTTATTTTTT TTAAAGCCTA AATATAACTT TTATTTATTT TTATGACATC CTTACTCAGG AAATGAGAGC TTTGTTGTGA AGTAAACAAC TAAAGCAACC AAAGAATTGA CATTTGTAGA CTAAAGCATC ACGTGATGAA AGTTGGATAG GCTCTCAATG CTGCTGGAGG CTAAGAGATT GGGACCGGGG CAAATGTTCA GAAGCTCTAA GAGCTTATTT GAAACTACCT CAGTTCTTCC TTACCACTGT GCATGGAGTG AAATATCAAA ATGTAGAGAC ATGCCTTTAG ACTCTGGTGA GGCATTTATC CTAGCCCTCC CTGCACCTGG CTGGTGTTTG AGTGGATTCA GATTTCTTGT CATTACCAAT ATCACAATCA TGACTATCTT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001001851, NM_030569, NM_032817
UniProt ID Q86UX2
Synonyms ITI-HC5; PP14776
Summary This gene encodes a heavy chain component of one of the inter-alpha-trypsin inhibitor (ITI) family members. ITI proteins are involved in extracellular matrix stabilization and in the prevention of tumor metastasis. They are also structurally related plasma serine protease inhibitors and are composed of a light chain and varying numbers of heavy chains. This family member is thought to function as a tumor suppressor in breast and thyroid cancers. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2011
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Documents
Resources
Other Versions
SKU Description Size Price
GA112972 ITIH5 CRISPRa kit - CRISPR gene activation of human inter-alpha-trypsin inhibitor heavy chain 5 1 kit
$1,657.00
KN214478BN ITIH5 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN214478LP ITIH5 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN214478RB ITIH5 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN414478 ITIH5 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

Inter-α-Trypsin Inhibitor Heavy Chain 5 (ITIH5) Is a Natural Stabilizer of Hyaluronan That Modulates Biological Processes in the Skin
Huth, Huth, Marquardt et al
Skin Pharmacol Physiol (2020)
Cited by 22
AI Summary: OriGene's ITIH5 Human Gene Knockout Kit via CRISPR (KN214478) was used to generate stable ITIH5 knockout KeratinoSens cells, enabling the study of ITIH5's role in hyaluronan stabilization and skin biology.
Snippet: mL G418. KeratinoSens cells with stable ITIH5 knockout were generated using the ITIH5 Human Gene Knockout Kit via CRISPR (KN214478;OriGene, Rockville, MD, USA), according to the manufacturer's instructions. As controls
Showing 1-1 of 1 papers.
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