TPR Human Gene Knockout Kit (CRISPR)

CAT#: KN214233

TPR - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: TPR
• Locus ID: 7175
• Components:

  KN214233G1, TPR gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGCGGTGTTGCAGCAAGTCC

  KN214233G2, TPR gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGTTTGTTCTGGACAGACTT

  KN214233D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  ATGCCCTCTA GTGGCTCAGT GTCCAGAAGC CGCCAACGAG GACGATCCAT GGCATCCCGG GGCTTTGACG GTAAGGTCAC CAGCTCTTCA GGGAGGGCCA AGGGGTGACG AGTACGTATG TGGCGCCACC GTGCGGACAG CGGTTACGGG GCCGAGATAG GAGAGCCAGG GGAGCGGGGA GAAAAACGGA AGCTGAGAGG GCGGTGCAGC ATTGGGCTCC CGGAAAAGAA TGAGAAAAAC ACTACATTTC CCAGAGCCCT ACAAGGCCTG AAGTGGGTGG AGCTGGAAGC CGGAAAATTG GGTGCGCAAG AGGATCAGGG ATAGCCTCTG AGCTCGGGTT CCCAGGGTTC GTAGCTTCCA ACGGCTGCGC GCGCACTTCG GTCGCGGGCG GTGAGGTGCT GTTGCTGAAA CGCTGCCGCT GAGGGTGGAC TCGATTTCCC AGGGTCCCGC CGCGGGAGTC TCCGGCGGGC GGGCGCGCGC GAGCCACCGA GCGAGGTGAT AGAGGCGGCG GCCCAGGCGT CTGGGTCCTG CTGGTCTTCG CCTTTCTTCT CCGCTTCTAC CCCGTCGGCC GCTGCCACTG GGGTCCCTGG CCCCACCGAC GAAAAGTTCC TTGCTGATCA GCAATCCGAG ATCGATGGCC TGAAGGGGCG GCATGAGAAA TTTAAGGTGG AGAGCGGTAA GTGAGATGCT CTTTCCGTCC TTGGTTTTGG GCTCGACTCC CGCCCTTTGG GTCTGGTCTT CCCCCATCAA GTTGGCACTG GGTACCGCTC TGTTAACCTC ACTTCCTGGG CTCTGTGGAT ATCCTGAGCC TTCGCTTTCC TGCTCCTTAC AGTTTGATAA GAAGCCTTTG AAAAAAAAAT CTGAATCTAA GTTCAGTAGC ATTTTCTTTG TAGTTAGGGT AGGCTCATAC AGGGACGCTT ATTTTCCTTC GTCCGGCAGA CGGGAAACAG ACTTGATAGA GTCTGCGAAC CCATCTCTCT AAGCCTAGAG TACCTTATAT GTTTTGTTGC ACTTCAACTG ATAGTGGCTC CGCCCTATTT TCTAGGCACC TGCATTCTAG GCCTTTGCCA AATTGACCAC TCTGTGCACT TGTTCTTAGT GTGTGCGTCG TCCCGTGGCT CCAGAACGAA TCAGATGTTT ACAAAAATGA ATATTAGGTG CATAAGAGCG CTGGAAACAC AGTGAATGTG CAGTACATAA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_003292
• UniProt ID: P12270
• Synonyms: nuclear pore complex-associated protein TPR; nucleoprotein TPR; OTTHUMP00000033584; translocated promoter region (to activated MET oncogene); tumor potentiating region
• Summary: This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq, Jul 2008]