TPR (NM_003292) Human Tagged ORF Clone

SKU RC214233

TPR (Myc-DDK-tagged)-Human translocated promoter region (to activated MET oncogene) (TPR). Note: ORF is codon optimized

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214233 ORF sequence, codon optimized.; Due to the complexity of NM_003292, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGCGGTCCTCCAGCAAGTGCTGGAGAGAACTGAACTGAATAAATTGCCCAAGTCCGTGCAGAATA; AGCTTGAAAAGTTCCTTGCAGATCAACAATCTGAGATCGACGGGTTGAAGGGTAGACACGAAAAGTTTAA; GGTTGAGTCAGAACAACAATACTTCGAGATCGAGAAAAGGCTGTCTCATTCTCAAGAGCGCTTGGTGAAC; GAGACACGCGAGTGTCAGTCCCTTAGACTTGAACTGGAGAAGCTCAATAACCAGTTGAAGGCACTGACAG; AAAAGAACAAAGAGCTGGAGATCGCACAAGACAGAAATATAGCCATTCAATCCCAGTTTACCAGAACAAA; GGAAGAGCTGGAAGCCGAGAAAAGAGACTTGATCCGCACTAACGAACGCTTGAGCCAGGAACTGGAGTAT; CTGACTGAGGATGTTAAAAGGCTGAACGAAAAGCTGAAGGAAAGCAATACGACGAAGGGAGAGCTGCAGC; TCAAATTGGACGAACTTCAGGCATCCGATGTGAGTGTCAAGTATAGGGAGAAACGCCTGGAGCAGGAGAA; GGAGTTGCTGCACTCCCAGAACACCTGGCTCAACACAGAGCTGAAAACAAAAACTGACGAGCTTCTCGCG; TTGGGTAGGGAAAAAGGCAACGAGATCCTTGAGCTCAAGTGTAACCTCGAGAATAAGAAGGAGGAGGTAT; CCCGCCTGGAGGAGCAGATGAACGGACTGAAAACTAGCAACGAACACTTGCAGAAACACGTCGAGGATCT; GCTCACCAAGCTGAAGGAGGCAAAAGAGCAGCAGGCCAGCATGGAGGAGAAGTTCCACAACGAGCTGAAC; GCTCACATTAAACTGAGCAACCTGTATAAATCCGCTGCCGATGATAGCGAGGCAAAAAGCAATGAACTCA; CTAGAGCCGTTGAGGAGCTGCACAAGCTGCTGAAAGAGGCGGGAGAGGCTAACAAGGCGATCCAAGATCA; TCTGTTGGAGGTGGAACAGAGTAAGGATCAAATGGAAAAGGAGATGTTGGAAAAAATTGGCCGCTTGGAG; AAAGAACTTGAAAATGCTAACGATCTCCTGTCCGCAACAAAGCGCAAGGGGGCCATTCTCTCTGAGGAGG; AGCTGGCCGCTATGTCCCCAACAGCCGCCGCGGTCGCAAAGATCGTCAAGCCTGGTATGAAGCTGACGGA; GCTGTATAACGCTTATGTGGAGACACAAGACCAGCTGCTGTTGGAAAAGTTGGAGAACAAGCGCATCAAT; AAGTATTTGGACGAAATTGTTAAGGAGGTGGAGGCCAAAGCGCCGATTTTGAAGCGGCAGAGAGAGGAGT; ACGAGAGAGCTCAAAAAGCAGTGGCATCCCTCTCAGTGAAGCTTGAACAGGCGATGAAAGAAATCCAGAG; ACTGCAGGAGGACACCGATAAAGCCAACAAACAGTCTTCCGTCCTGGAGCGAGATAACCGGAGGATGGAA; ATCCAGGTGAAGGACCTCTCTCAGCAGATTCGGGTGCTTCTGATGGAACTGGAGGAGGCAAGGGGGAATC; ACGTCATTAGGGACGAGGAGGTGAGCTCCGCCGACATCAGTTCTTCAAGCGAAGTTATTAGCCAACACTT; GGTATCCTACAGGAATATTGAGGAGTTGCAACAGCAGAACCAGAGGCTGCTGGTGGCTCTTCGCGAACTG; GGGGAGACTCGCGAACGCGAGGAACAGGAGACTACCTCTTCCAAGATAACTGAGCTGCAGTTGAAGCTCG; AGTCCGCCCTCACCGAGCTTGAGCAGCTGCGGAAGTCAAGACAGCACCAGATGCAGCTTGTTGACTCCAT; CGTCCGGCAAAGGGACATGTACCGCATCCTGCTGAGTCAGACCACAGGGGTAGCTATCCCCCTGCATGCC; AGTAGTCTGGATGACGTCTCTCTGGCCAGTACCCCAAAGAGGCCCTCTACCAGTCAAACCGTCAGTACCC; CTGCTCCGGTGCCTGTTATCGAGAGCACAGAGGCAATTGAGGCGAAGGCAGCTCTTAAGCAGCTCCAGGA; AATATTCGAAAACTACAAGAAGGAGAAAGCCGAAAACGAAAAGATTCAAAACGAGCAGCTCGAGAAGCTC; CAGGAGCAAGTGACTGATCTCCGCTCTCAGAACACTAAAATATCCACACAATTGGACTTCGCCTCCAAGC; GGTATGAGATGCTTCAAGACAACGTGGAGGGTTACAGACGAGAGATTACCAGCCTGCATGAGAGGAACCA; AAAGCTGACAGCCACCACCCAGAAACAAGAGCAAATCATTAACACCATGACGCAAGACCTGAGGGGCGCG; AACGAGAAGCTGGCGGTGGCCGAGGTCCGCGCAGAGAACCTTAAGAAGGAGAAAGAGATGCTTAAACTTA; GCGAGGTCAGACTGAGCCAGCAACGCGAAAGCCTGCTGGCTGAACAGAGGGGCCAGAATCTCCTCCTGAC; TAATCTGCAAACAATTCAGGGTATCTTGGAGAGGTCCGAAACTGAAACAAAGCAACGGCTTTCAAGCCAG; ATTGAAAAATTGGAGCACGAAATTAGCCACCTCAAGAAGAAGCTGGAGAATGAGGTGGAGCAGCGACACA; CCCTCACCCGGAATCTCGACGTCCAGCTGCTTGACACTAAAAGGCAACTGGATACAGAAACCAACCTGCA; CCTTAATACTAAGGAGCTGCTGAAGAACGCACAGAAAGAAATTGCTACTCTTAAGCAGCACCTCTCAAAT; ATGGAGGTCCAGGTAGCCTCACAATCTTCACAGCGGACCGGAAAAGGGCAGCCGTCCAACAAAGAGGACG; TGGATGACCTCGTGTCCCAGCTGCGGCAGACAGAAGAGCAGGTGAACGACCTCAAGGAACGCCTTAAAAC; TTCCACTAGTAACGTTGAACAGTATCAAGCGATGGTTACCAGTCTGGAAGAGTCACTGAACAAAGAAAAG; CAGGTGACCGAGGAGGTCAGGAAAAACATCGAGGTGCGCCTGAAAGAGTCTGCAGAATTCCAGACCCAGC; TGGAGAAGAAACTGATGGAGGTGGAGAAGGAGAAGCAGGAGCTCCAGGATGATAAAAGGCGGGCCATCGA; GTCTATGGAGCAGCAACTGTCCGAACTCAAAAAAACCTTGTCTTCTGTGCAGAATGAAGTGCAAGAGGCG; TTGCAGAGAGCATCCACCGCTCTGAGTAATGAACAGCAGGCCAGACGGGACTGTCAGGAACAAGCCAAGA; TCGCAGTCGAAGCTCAGAACAAGTACGAACGCGAACTGATGCTTCATGCAGCAGACGTGGAAGCACTGCA; AGCCGCAAAAGAGCAGGTGTCCAAGATGGCCAGCGTACGGCAACATTTGGAAGAGACAACACAAAAGGCA; GAGAGCCAGCTTTTGGAATGCAAGGCCAGCTGGGAGGAGCGCGAGAGAATGCTTAAGGACGAAGTGTCAA; AGTGTGTGTGCAGATGCGAGGACCTGGAGAAACAAAATCGACTCCTCCACGATCAGATCGAGAAACTGTC; CGATAAAGTTGTTGCATCAGTGAAGGAGGGGGTGCAGGGCCCTCTGAATGTGTCCCTGTCCGAGGAGGGC; AAAAGTCAAGAGCAGATACTCGAAATACTCAGGTTCATCAGGCGGGAGAAAGAAATTGCAGAGACAAGAT; TTGAGGTGGCCCAGGTGGAAAGTCTGCGATATAGACAAAGGGTCGAGCTCCTCGAACGCGAGCTGCAAGA; ACTCCAAGATAGTCTCAACGCAGAAAGAGAGAAGGTCCAAGTTACTGCCAAAACAATGGCCCAGCACGAG; GAGCTGATGAAGAAAACGGAAACGATGAATGTCGTCATGGAGACAAATAAGATGCTGAGAGAGGAAAAAG; AGCGCCTGGAACAAGATCTCCAGCAAATGCAGGCGAAAGTTCGAAAACTGGAGCTTGACATCCTCCCCCT; TCAGGAAGCAAACGCGGAGCTCTCAGAAAAAAGCGGCATGTTGCAAGCCGAAAAGAAATTGCTCGAAGAA; GACGTGAAACGCTGGAAGGCTAGGAACCAACACCTGGTGAGCCAGCAAAAGGATCCGGATACCGAAGAAT; ATCGGAAGTTGTTGTCAGAGAAGGAGGTTCACACCAAGCGCATTCAACAGCTTACCGAAGAAATAGGGAG; ACTCAAGGCCGAGATCGCTCGCTCAAACGCCTCACTCACTAACAACCAAAACCTCATACAGAGTCTGAAA; GAGGACCTGAATAAAGTGCGAACGGAAAAAGAAACTATCCAAAAGGATTTGGACGCAAAGATCATCGATA; TCCAGGAGAAGGTAAAAACTATCACACAGGTCAAAAAGATTGGTCGCCGCTATAAGACACAGTATGAGGA; ACTGAAAGCTCAACAGGACAAGGTAATGGAGACGTCTGCACAATCCTCCGGGGATCATCAGGAGCAGCAC; GTTTCTGTGCAGGAGATGCAGGAGCTGAAAGAGACTCTGAATCAAGCGGAAACCAAGTCCAAGAGCCTGG; AGTCCCAGGTTGAGAACCTTCAGAAGACTCTTTCTGAGAAAGAAACAGAAGCTAGGAACTTGCAGGAGCA; GACAGTCCAGTTGCAAAGCGAGCTGAGCCGCCTCAGACAGGACCTGCAGGATCGCACCACTCAGGAAGAG; CAGCTTCGGCAGCAGATCACCGAAAAAGAGGAGAAGACACGCAAGGCAATTGTAGCAGCAAAGAGCAAGA; TCGCTCACTTGGCTGGCGTGAAAGACCAACTCACAAAGGAAAACGAGGAGTTGAAGCAGCGAAATGGAGC; CTTGGATCAGCAGAAGGATGAGCTCGACGTGCGGATCACGGCCTTGAAAAGCCAGTATGAGGGGCGCATC; TCAAGACTGGAACGCGAGCTGAGGGAACACCAGGAACGCCACCTGGAGCAGAGGGACGAACCACAGGAAC; CGTCTAACAAAGTACCGGAGCAGCAGAGGCAGATAACCCTGAAGACCACCCCGGCATCCGGGGAGCGGGG; AATCGCTTCAACTAGCGACCCTCCTACCGCGAACATAAAGCCCACACCAGTCGTTTCCACACCTAGTAAG; GTGACGGCTGCCGCAATGGCCGGCAATAAAAGTACGCCAAGGGCCTCAATCCGGCCCATGGTGACCCCTG; CAACGGTCACTAACCCCACAACAACACCCACCGCCACCGTAATGCCAACCACCCAAGTGGAATCACAGGA; GGCCATGCAGAGCGAAGGCCCAGTGGAGCACGTGCCAGTCTTTGGCAGCACGTCTGGCTCTGTTCGGTCA; ACGAGCCCGAATGTTCAGCCATCAATCTCACAGCCCATCCTGACTGTACAGCAGCAAACGCAGGCAACCG; CCTTTGTCCAGCCTACTCAGCAGTCACATCCTCAGATCGAGCCGGCCAATCAAGAGCTTAGTTCCAATAT; TGTAGAGGTGGTGCAGAGCTCTCCCGTGGAGAGGCCGTCTACATCCACGGCAGTCTTTGGGACCGTGAGC; GCCACACCTAGTTCTTCTTTGCCTAAGCGGACCAGAGAAGAGGAGGAGGACAGTACTATAGAAGCTTCTG; ATCAAGTAAGCGATGACACGGTGGAAATGCCGCTTCCCAAGAAGCTGAAGTCTGTCACCCCAGTGGGTAC; CGAAGAAGAGGTCATGGCAGAGGAGAGCACCGATGGAGAGGTGGAGACACAGGTGTACAATCAGGATTCC; CAAGACTCCATTGGTGAGGGCGTCACTCAGGGCGACTATACACCAATGGAGGATTCTGAGGAGACATCCC; AATCTCTGCAGATAGACCTGGGCCCGCTCCAGAGTGACCAGCAAACAACCACATCCTCTCAGGACGGACA; GGGCAAAGGAGACGACGTTATCGTCATTGATAGCGATGATGAAGAAGAGGATGATGATGAGAATGATGGC; GAACATGAGGACTATGAAGAGGACGAGGAGGATGACGACGATGATGAAGACGATACAGGGATGGGAGACG; AGGGGGAAGATAGCAACGAAGGCACAGGTAGTGCCGATGGAAATGACGGATATGAAGCTGATGACGCTGA; GGGCGGAGATGGTACAGATCCCGGCACAGAGACAGAGGAGAGCATGGGTGGGGGCGAAGGGAATCATCGG; GCAGCTGACTCACAGAATAGCGGCGAAGGCAATACAGGGGCTGCAGAAAGTTCTTTTTCCCAGGAAGTAT; CACGAGAGCAACAGCCATCCTCTGCCAGCGAACGCCAGGCCCCTAGAGCTCCCCAGTCACCTAGGAGGCC; TCCTCACCCACTGCCCCCCAGACTCACAATCCACGCACCCCCCCAAGAACTGGGTCCTCCCGTCCAGAGA; ATCCAGATGACCCGGCGGCAGTCAGTAGGAAGGGGCCTGCAACTCACGCCTGGAATAGGCGGGATGCAAC; AGCATTTTTTTGATGACGAGGACCGCACCGTGCCTTCAACACCAACCCTGGTGGTACCGCACCGAACCGA; TGGATTCGCCGAGGCAATACATTCTCCTCAGGTAGCAGGAGTGCCAAGATTCAGATTTGGTCCTCCAGAA; GACATGCCTCAAACAAGCAGTTCTCATAGCGATCTCGGACAGCTTGCAAGCCAGGGCGGGTTGGGGATGT; ACGAAACCCCATTGTTTCTGGCTCACGAAGAGGAGAGCGGAGGCAGAAGCGTGCCCACTACACCTTTGCA; GGTGGCCGCCCCCGTCACTGTCTTCACTGAAAGCACCACCTCTGACGCTAGCGAACACGCTTCCCAAAGT; GTTCCCATGGTAACAACCAGCACCGGCACACTTAGTACAACGAATGAAACCGCGACAGGCGACGACGGAG; ATGAGGTGTTTGTGGAGGCCGAATCTGAAGGTATTTCATCAGAGGCCGGATTGGAAATTGATTCCCAGCA; GGAGGAGGAGCCAGTTCAAGCTAGCGACGAGTCCGATCTGCCATCCACTAGTCAGGACCCACCGTCAAGC; TCCTCAGTAGATACATCATCCAGCCAGCCCAAGCCGTTCAGAAGAGTGCGGCTCCAGACCACACTTCGGC; AAGGCGTGAGGGGGCGGCAGTTCAACCGACAGCGAGGTGTTTCTCATGCCATGGGCGGGAGAGGAGGAAT; TAACAGGGGAAATATCAAT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214233 representing NM_003292; Red=Cloning site Green=Tags(s); ; MAAVLQQVLERTELNKLPKSVQNKLEKFLADQQSEIDGLKGRHEKFKVESEQQYFEIEKRLSHSQERLVN; ETRECQSLRLELEKLNNQLKALTEKNKELEIAQDRNIAIQSQFTRTKEELEAEKRDLIRTNERLSQELEY; LTEDVKRLNEKLKESNTTKGELQLKLDELQASDVSVKYREKRLEQEKELLHSQNTWLNTELKTKTDELLA; LGREKGNEILELKCNLENKKEEVSRLEEQMNGLKTSNEHLQKHVEDLLTKLKEAKEQQASMEEKFHNELN; AHIKLSNLYKSAADDSEAKSNELTRAVEELHKLLKEAGEANKAIQDHLLEVEQSKDQMEKEMLEKIGRLE; KELENANDLLSATKRKGAILSEEELAAMSPTAAAVAKIVKPGMKLTELYNAYVETQDQLLLEKLENKRIN; KYLDEIVKEVEAKAPILKRQREEYERAQKAVASLSVKLEQAMKEIQRLQEDTDKANKQSSVLERDNRRME; IQVKDLSQQIRVLLMELEEARGNHVIRDEEVSSADISSSSEVISQHLVSYRNIEELQQQNQRLLVALREL; GETREREEQETTSSKITELQLKLESALTELEQLRKSRQHQMQLVDSIVRQRDMYRILLSQTTGVAIPLHA; SSLDDVSLASTPKRPSTSQTVSTPAPVPVIESTEAIEAKAALKQLQEIFENYKKEKAENEKIQNEQLEKL; QEQVTDLRSQNTKISTQLDFASKRYEMLQDNVEGYRREITSLHERNQKLTATTQKQEQIINTMTQDLRGA; NEKLAVAEVRAENLKKEKEMLKLSEVRLSQQRESLLAEQRGQNLLLTNLQTIQGILERSETETKQRLSSQ; IEKLEHEISHLKKKLENEVEQRHTLTRNLDVQLLDTKRQLDTETNLHLNTKELLKNAQKEIATLKQHLSN; MEVQVASQSSQRTGKGQPSNKEDVDDLVSQLRQTEEQVNDLKERLKTSTSNVEQYQAMVTSLEESLNKEK; QVTEEVRKNIEVRLKESAEFQTQLEKKLMEVEKEKQELQDDKRRAIESMEQQLSELKKTLSSVQNEVQEA; LQRASTALSNEQQARRDCQEQAKIAVEAQNKYERELMLHAADVEALQAAKEQVSKMASVRQHLEETTQKA; ESQLLECKASWEERERMLKDEVSKCVCRCEDLEKQNRLLHDQIEKLSDKVVASVKEGVQGPLNVSLSEEG; KSQEQILEILRFIRREKEIAETRFEVAQVESLRYRQRVELLERELQELQDSLNAEREKVQVTAKTMAQHE; ELMKKTETMNVVMETNKMLREEKERLEQDLQQMQAKVRKLELDILPLQEANAELSEKSGMLQAEKKLLEE; DVKRWKARNQHLVSQQKDPDTEEYRKLLSEKEVHTKRIQQLTEEIGRLKAEIARSNASLTNNQNLIQSLK; EDLNKVRTEKETIQKDLDAKIIDIQEKVKTITQVKKIGRRYKTQYEELKAQQDKVMETSAQSSGDHQEQH; VSVQEMQELKETLNQAETKSKSLESQVENLQKTLSEKETEARNLQEQTVQLQSELSRLRQDLQDRTTQEE; QLRQQITEKEEKTRKAIVAAKSKIAHLAGVKDQLTKENEELKQRNGALDQQKDELDVRITALKSQYEGRI; SRLERELREHQERHLEQRDEPQEPSNKVPEQQRQITLKTTPASGERGIASTSDPPTANIKPTPVVSTPSK; VTAAAMAGNKSTPRASIRPMVTPATVTNPTTTPTATVMPTTQVESQEAMQSEGPVEHVPVFGSTSGSVRS; TSPNVQPSISQPILTVQQQTQATAFVQPTQQSHPQIEPANQELSSNIVEVVQSSPVERPSTSTAVFGTVS; ATPSSSLPKRTREEEEDSTIEASDQVSDDTVEMPLPKKLKSVTPVGTEEEVMAEESTDGEVETQVYNQDS; QDSIGEGVTQGDYTPMEDSEETSQSLQIDLGPLQSDQQTTTSSQDGQGKGDDVIVIDSDDEEEDDDENDG; EHEDYEEDEEDDDDDEDDTGMGDEGEDSNEGTGSADGNDGYEADDAEGGDGTDPGTETEESMGGGEGNHR; AADSQNSGEGNTGAAESSFSQEVSREQQPSSASERQAPRAPQSPRRPPHPLPPRLTIHAPPQELGPPVQR; IQMTRRQSVGRGLQLTPGIGGMQQHFFDDEDRTVPSTPTLVVPHRTDGFAEAIHSPQVAGVPRFRFGPPE; DMPQTSSSHSDLGQLASQGGLGMYETPLFLAHEEESGGRSVPTTPLQVAAPVTVFTESTTSDASEHASQS; VPMVTTSTGTLSTTNETATGDDGDEVFVEAESEGISSEAGLEIDSQQEEEPVQASDESDLPSTSQDPPSS; SSVDTSSSQPKPFRRVRLQTTLRQGVRGRQFNRQRGVSHAMGGRGGINRGNIN; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003292
• ORF Size: 7089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003292.2, NP_003283.2
• RefSeq Size: 9636 bp
• RefSeq ORF: 7092 bp
• Locus ID: 7175
• UniProt ID: P12270
• Cytogenetics: 1q31.1
• Domains: M, Pox_A_type_inc
• Protein Pathways: Pathways in cancer, Thyroid cancer
• MW: 267.3 kDa
• Summary: This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq, Jul 2008]