PLAC8 Human Gene Knockout Kit (CRISPR)

SKU KN212588BN

PLAC8 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Vector: mBFP-Neo
• Target Symbol: PLAC8
• Locus ID: 51316
• Components:

  KN212588G1, PLAC8 gRNA vector 1 in pCas-Guide CRISPR vector

  KN212588G2, PLAC8 gRNA vector 2 in pCas-Guide CRISPR vector

  KN212588BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  Homologous arm and mBFP-Neo sequences:

  pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet

  TCCCGGTTCA AGCGATTCTC CTGCCTCGGC CTCCCGAGTA GCTGGAGTTA CAGGCACGCG CCACCAAGCC TGGCTAATTT TTTGCATTTT TAGTAGAGGC GGGGTTTCGC CATACTGGCC AGGCTGGTCT CGAATTCCTG ACCTCAAATG ATCCGTCGGC CTCGGCCTCC CAAAGTGCCG GGATTACAGA CCTGAGTCAC CGCGCCCGGC CCAGTTCGGT GAATTCCTTC CCCTCACCCC CAGGGTCTCT ATCTTGTGAA GGGTGGAGAT AGAAATTCTG TTCTTCAAAC AGAGCAACTT CCTCTACACT GCCTCAGCAT CCTGCCCCTA AAGTGGGCCT TTAAAGAGCA ACCTCGAGAT TTCTTTTATA TATATATATA TATTTTATTA TACTTTAAGT TCTAGGGTAC ATGTGCACAA CGTGCAGGTT TGTTACATAT GTATACATGT GCCATGTTGG TGTGCTGCAC CCATTAACTC GTCATTTACA TTAGGTATAT CTCCTAATGC TATCCCTCTC CCCTCCCCCC ACCCCACAAC AGAGATTTCT AACACCACTC TGCCTTTTCA GTCCACTGCT TTTAAGGCCC TGCACTGAAA GCATGTGTGA CTGTTTCAGC GACTGCGGAG TCTGTAAGTG TGTGGGGAAA GAACCCCCTT ATTTGATACC GAGGTGAATT ACCAATGACT CAATCCTTCC CCCATTCCTC CCTTATTGTG TAGAAAAAGT TTCTAACTTA TTACAAGCAC TCTGCATGTC AGGCAATGGT ATGTTTAGCA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAGCCT CCGTATGGAT AACTTAATTT TCTCCAGCCC TTCAAAGAAC AGTCTTACTC CTAAAATAAG TTCTGGGTTA AGATAATAGC AAAAGAGCTG ATTGATTCTA ACAACGAGCA GGTCTGGCCT GACTAAATAG AACTTACATT AATTTAAAAA TTCCAAGTTG AACTTGATTT TTTTAGAAAG ACTGTTCTTG CAACATATAA ATTAACCTCA TTAATTTATA GTTACATTGT GTCCAGTACC CAAGGGTTAG ATTCATGGTT TCCATATGCG TTCAGAGAAA ACTATTTTAA TGTATATATT TAGGTTTTTT ACACACCTGA GAATAAAGAA TTCTGGAGGC AATTAGCTTG TCACTGGGGA AAAAAAATCT CTGACTGCTT

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001130715, NM_001130716, NM_016619
• UniProt ID: Q9NZF1
• Synonyms: C15; DGIC; onzin; PNAS-144
• Shipping: Ambient