Eph receptor A4 (EPHA4) Human Gene Knockout Kit (CRISPR)

CAT#: KN211230

EPHA4 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Eph receptor A4
• Locus ID: 2043
• Components:

  KN211230G1, Eph receptor A4 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGGGTATACCCCGCGAATGA

  KN211230G2, Eph receptor A4 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGGATTTGCGACGCTGTCAC

  KN211230D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GGGGAAGCGC CATAAAGACA AAACAGGGCA AGGTGGCAGA GGGTGGCGGT GGCAGGCGCG AGTCTGCAGC TCAGCCCGGG CGCGCTGGAC GCAGACACGT CGACTCTCCT AAAGAAGCAA CCGCAGGTCA GCTTAGACTT CTTTTATTTC AATAAAAGAG CGTTTTCCTC CATCTTAAAC CGCCCGTGGT GGGAGAAAAG GTTTCCAACC TCTTCTCTGG CTCCCCTCGG TCAATTCCGA GCTTTTCCAA AGTCCCTCCG CCGTCCAGGG GCTCGACCTT CGAGGTAACG TGGGGGATCC CGCTGGCTCC CTCCGGCAGG CGGGGGCGGG GACCGGCCGT GCTCGCCCCC TAGAGCCCCG GGAGACCGCG CGCCCCGTGG AGCCCGGAGG GGGCGGAACC CTGGGGCGGG CCCTGCTCGG TGAAGGCGGG GCTCGGCCGC CCTGTCTGCG CCGCCATTGG CCGCGGGCCC CGGACTGGCG GGCTCACGTC ACCGGCCGGG CCCGCCCCCT TGGCAATGTC TTCAGCCCGC TGCTCTCCTA ACTTAAGCAT TTATTCCACT GGGATAGAAG CGGCAGGAGC AGCGTTGGCA CCGGCGAACC AGGTAAGAGC GGGACGGCCT TTCTGTGGTC GGGGACAGCG AGGGGCCATC GCGGGTGAGG GGTCCTCAGG AGCGCAGGGC CAGGTGGTGC GGGGGGAGCC CAGTGTGAAT GGTGCGTCCC GATTCGGGAG AAGGGGGCTG AGGCGAGGGG ATCGGCGAAG GGGCAGCGGG GAACCGCCGG ACAGCCTGCC CCGAGGCGGT GACCCGTGCA CGCGGCGGGT CTGTCCAAGT TTGTAACCAA AGGCCAAAGT TTATCAGCAT CCGCCAGAGA GGCCCGCTCG AGGCGCCCTG AGGGGATGCA CGGGAGACAA CGCCGGGATT TAAAAAAAAA ATTACAAGAA CCACGTCCTC TCCTGAGCCA GCCCGGGGCG AGGGGAAAAC GCAGCCTGGA GCACAGTGAA GGCTTGGTGC TCCCGGATTC TAGCGAAAGC CTTTCGAGCT CTGGTTTCTG CAAAGTTTCC CGAGCCCTGC GCTTCTCGGG ACCGGGCTGC GGGGCCTGCT CCGCAGCGCA GCGGTCGTGG CGCCGCTCGT CAGGCGCCAG TGACCTGATT TCTAGCACCC GGAGTCAGAC ACCTTTCTCC TCGCCAAGCC CAGCGCCCCG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001304536, NM_001304537, NM_004438, NM_001363748
• UniProt ID: P54764
• Synonyms: HEK8; SEK; TYRO1
• Summary: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]