Eph receptor A4 (EPHA4) (NM_004438) Human Tagged ORF Clone
$1,354.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Eph receptor A4 |
| Synonyms | EK8; HEK8; SEK; TYRO1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004438 |
| ORF Size | 2958 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004438.5 |
| RefSeq Size | 6364 bp |
| RefSeq ORF | 2961 bp |
| Locus ID | 2043 |
| UniProt ID | P54764 |
| Cytogenetics | 2q36.1 |
| Domains | EPH_lbd, FN3, pkinase, SAM, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 109.7 kDa |
| Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004438" in other vectors (8)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211230L1 | Lenti-ORF clone of EPHA4 (Myc-DDK-tagged)-Human EPH receptor A4 (EPHA4) | 10 ug |
$1,654.00
|
|
| RC211230L2 | Lenti-ORF clone of EPHA4 (mGFP-tagged)-Human EPH receptor A4 (EPHA4) | 10 ug |
$1,654.00
|
|
| RC211230L3 | Lenti-ORF clone of EPHA4 (Myc-DDK-tagged)-Human EPH receptor A4 (EPHA4) | 10 ug |
$1,654.00
|
|
| RC211230L4 | Lenti-ORF clone of EPHA4 (mGFP-tagged)-Human EPH receptor A4 (EPHA4) | 10 ug |
$1,654.00
|
|
| RC600041 | EPHA4 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor A4, Signal peptide (1-19) plus EC domain (20-547) | 10 ug |
$764.00
|
|
| RG211230 | EPHA4 (tGFP-tagged) - Human EPH receptor A4 (EPHA4) | 10 ug |
$1,554.00
|
|
| SC117354 | EPHA4 (untagged)-Human EPH receptor A4 (EPHA4) | 10 ug |
$1,355.00
|
|
| SC323668 | EPHA4 (untagged)-Kinase deficient mutant (K653M) of Human EPH receptor A4 (EPHA4) | 10 ug |
$1,355.00
|
|
Citations
Establishment of a Novel Anti-EphA4 Monoclonal Antibody, Ea4 Mab-3, for Versatile Applications
Saga, Li, Tanaka et al
preprints.org (2025)
Saga, Li, Tanaka et al
preprints.org (2025)
AI Summary: OriGene’s EphA4 cDNA (Catalog No.: RC211230) was used as a template for generating tagged expression constructs, which facilitated the development and characterization of the novel anti-EphA4 monoclonal antibody described in the study.
Snippet: from the Cell Resource Center at Tohoku University (Miyagi, Japan). The complementary DNA of EphA4 (Catalog No.: RC211230, OriGene Technologies, Inc., Rockville, MD, USA) plus an N-terminal MAP16 tag and an N-terminal PA16 tag were subcloned into a pCAG-Ble vector [FUJIFILM Wako Pure Chemical Corporation (Wako), Osaka, Japan]. …
Identification and Characterisation of Nanobodies targeting the EphA4 receptor
Schoonaert, Rué, Roucourt et al
J. Biol. Chem. (2017)
Schoonaert, Rué, Roucourt et al
J. Biol. Chem. (2017)
Showing 1-2 of 2 papers.
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