GPR 174 (GPR174) Human Gene Knockout Kit (CRISPR)

CAT#: KN211205

GPR174 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: GPR 174
• Locus ID: 84636
• Components:

  KN211205G1, GPR 174 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCTAATTACACGTGTACCAG

  KN211205G2, GPR 174 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TACACTGTCATTCTTGTGCC

  KN211205D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AAGCTGGGCC AGGAAATAAT TGACTTATTT TCTTTTGCTT GCAGCTCTGC CTTTGTTTCC TACTTTTTAG CTTTTGACCT ATAGCTGAAA AAGTCCCAGA AAGCTGATCT TTCCAAGTTA ATCATCCCTG AAGAGTGGCG GACTCATTGT GCACTAAAGC AATCCTTTCT TCTATGTGAG CCAAAAAAAG AAAAACGGCC TGCCAGACAC CTGTAAAGAG AAGGCTATCG TCATATAGGA ATTTGAGGAA CTGCTATATG GTACATTCCT TTTCCAAATG GGAGAGAATA AGCTCTCATG ATGTCCCAGA GGGCCTTAAA ATAAACAAGA GGGGAAATTG TAACAGCTTG TCATCTGTGC TTGTACACTA GACTTCCATG TAGTTACTCT AGAGAAATCT AAATCAAAAA TGCAGATCAT TCTTTGAAAA ATCCCCAAAT CATACGTAGA GACTCAGACA GATGTGGTAA AAAGAGGAAG GTTATTTTAT ACTTCGTATC TCCAACCCAC TGGCAATCAA TCTTTTGGAA GGAACAGCAG TTGATTGTGA ATTTAGTTTT GAACCACCAT TAGGCAAAGA TAGTTTCTCT AGAGAGAATC TCATAGGGAA TATATTAGCC CTGTGGGTAT TCTATGGTTA TATGAAAGAA ACAAAACGAG CTGTGATATT TATGATAAAC TTAGCCATTG CTGACTTACT ACAAGTTCTT TCCTTGCCAC TGAGGATCTT CTACTACTTG AATCATGACT GGCCATTTGG GCCTGGTCTC TGCATGTTCT GTTTCTACCT GAAGTATGTC AACATGTATG CAAGCATCTA CTTCTTGGTC TGCATCAGTG TGCGACGATT TTGGTTTCTC ATGTACCCCT TTCGCTTCCA TGACTGCAAA CAGAAATATG ACCTGTACAT CAGCATTGCT GGCTGGCTGA TCATCTGCCT TGCCTGTGTA CTCTTTCCAC TCCTCAGAAC CAGTGATGAT ACCTCTGGCA ATAGGACCAA ATGCTTTGTG GATCTTCCTA CCAGGAATGT CAACCTGGCC CAGTCCGTTG TTATGATGAC CATTGGCGAG TTGATTGGGT TTGTAACTCC GCTTCTGATT GTCCTATATT GTACCTGGAA GACGGTTTTA TCACTGCAAG ATAAATATCC CATGGCCCAA GATCTTGGAG AGAAACAGAA AGCCTTGAAG ATGATTCTAA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_032553
• UniProt ID: Q9BXC1
• Synonyms: FKSG79; GPCR17; LYPSR3
• Summary: This gene encodes a protein belonging to the G protein-coupled receptor superfamily. These proteins are characterized by the presence of seven alpha-helical transmembrane domains, and they activate or interact with various endogenous or exogenous ligands, including neurotransmitters, hormones, and odorant and taste substances. This family member is classified as an orphan receptor because the cognate ligand has not been identified. [provided by RefSeq, Sep 2011]