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SMURF 2 (SMURF2) Human Gene Knockout Kit (CRISPR)

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SKU
KN210866RB
SMURF2 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

Frequently Bought Together (3)

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Other products for "SMURF 2 or NM_022739.4"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Vector RFP-BSD
Target Symbol SMURF 2
Locus ID 64750
Components

KN210866G1, SMURF 2 gRNA vector 1 in pCas-Guide CRISPR vector

KN210866G2, SMURF 2 gRNA vector 2 in pCas-Guide CRISPR vector

KN210866RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_022739
UniProt ID Q9HAU4
Synonyms DKFZp686F0270; MGC138150
Summary E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.UniProtKB/Swiss-Prot Function
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Documents
Resources
Other Versions
SKU Description Size Price
GA112118 SMURF2 CRISPRa kit - CRISPR gene activation of human SMAD specific E3 ubiquitin protein ligase 2 1 kit
$1,657.00
KN210866 SMURF2 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN210866BN SMURF2 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN210866LP SMURF2 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN410866 SMURF2 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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