TXNIP Human Gene Knockout Kit (CRISPR)

CAT#: KN210804

TXNIP - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: TXNIP
• Locus ID: 10628
• Components:

  KN210804G1, TXNIP gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCTGAAAAGGTGTACGGCAG

  KN210804G2, TXNIP gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGCTGGCCGGGTGATAGTGG

  KN210804D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GACCCGGGAG AAGACGCTCA ACAGCCCCCT CCTTCCCCTC CTTCCTCTCC TTCCTCTCCT TCCCCCCTCC CTGCGCCGCT CCAGAGCGCA ACAACCATTT TCCCAGCCAG GAGCACACCG TGTCCACGCG CCACAGCGAT CTCACTGATT GGTCGGGCTC CTGGTAAACA AGGACCGGGC AGCCAATGGG AGGGATGTGC ACGAGGGCAG CACGAGCCTC CGGGCCAGCG CTCGCGTGGC TCTTCTGGCC CGGGCTACTA TATAGAGACG TTTCCGCCTC CTGCTTGAAA CTAACCCCTC TTTTTCTCCA AAGGAGTGCT TGTGGAGATC GGATCTTTTC TCCAGCAATT GGGGGAAAGA AGGCTTTTTC TCTGAATTAG CTTAGTGTAA CCAGCGGCGT ATATTTTTTA GGCGCCTTTT CGAAAACCTA GTAGTTAATA TTCATTTGTT TAAATCTTAT TTTATTTTTA AGCTCAAACT GCTTAAGAAT ACCTTAATTC CTTAAAGTGA AATAATTTTT TGCAAAGGGG TTTCCTCGAT TTGGAGCTTT TTTTTTCTTC CACCGTCATT TCTAACTCTT AAAACCAACT CAGTTCCATC GGCTTGCGGA GTGGCTAAAG TGCTTTGGAT GCAGGGATCC CAGCAGTGCA AACAGACTTC GGAGTACCTG CGCTATGAAG ACACGCTTCT TCTGGAAGAC CAGCCAACAG GTAAGCGGCC CAATTCATTG TTGGAGGGTG AAAGCTGATT AGAGAAGAGA ATTGAATACA CAAAACCTGT ACGAAATGTT TTAAGTTGCT CAGTTTGAGT GGTTTGAATT ACGTGTTGTT GCTTCCTTTT TTCTGTTTTA ATTTGCAGAC ATTCTCCTCC CCCCCCAAAA AAAAGGGTGA TTTGTACAAT TTTTTATGGT GCTGTGTCCT AAAGGGGATC CTGAGGGGCG TTGCCTCGGG TAGTTAAAGT CTTATGTGTG CATAAGTTGC TTATTCTTTG TCTACTTCCT ATTTGAGATG TTAGTAGAGA ACTGTCCTGG GTGAATCTTT CAGTATTGCA GGGCTTGGCA ACTTGCTGCC CGACAAAATA CATCAGAATT TCTCTTTAAG AACAATATGG GATGGATTAA AAAATATATA TATGGGATGA AATTGGGGGT ACTTCAATAC CTTGCATGCC ACCCAAGCAT TCCTTATCAC ACAGATGCAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001313972, NM_006472
• UniProt ID: Q9H3M7
• Synonyms: ARRDC6; EST01027; HHCPA78; THIF; VDUP1
• Summary: This gene encodes a thioredoxin-binding protein that is a member of the alpha arrestin protein family. Thioredoxin is a thiol-oxidoreductase that is a major regulator of cellular redox signaling which protects cells from oxidative stress. This protein inhibits the antioxidative function of thioredoxin resulting in the accumulation of reactive oxygen species and cellular stress. This protein also functions as a regulator of cellular metabolism and of endoplasmic reticulum (ER) stress. This protein may also function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]