Visfatin (NAMPT) Human Gene Knockout Kit (CRISPR)

SKU KN210707BN

NAMPT - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Vector: mBFP-Neo
• Target Symbol: Visfatin
• Locus ID: 10135
• Components:

  KN210707G1, Visfatin gRNA vector 1 in pCas-Guide CRISPR vector

  KN210707G2, Visfatin gRNA vector 2 in pCas-Guide CRISPR vector

  KN210707BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_005746, NM_182790
• UniProt ID: P43490
• Synonyms: 1110035O14Rik; PBEF; PBEF1; VF; VISFATIN
• Summary: This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]
• Shipping: Ambient