ATG5 Human Gene Knockout Kit (CRISPR)

CAT#: KN210563

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ATG5 - human gene knockout kit via CRISPR, HDR mediated

HDR-mediated knockout kit validation

  See Other Versions

USD 1,290.00

2 Weeks

    • 1 kit

Product images


Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol ATG5
Locus ID 9474
Kit Components

KN210563G1, ATG5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AACTTGTTTCACGCTATATC

KN210563G2, ATG5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AAGATGTGCTTCGAGATGTG

KN210563-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet


GE100003, scramble sequence in pCas-Guide vector

Disclaimer The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001286106, NM_001286107, NM_001286108, NM_001286111, NM_004849, NR_104402, NR_104403
Synonyms APG5; APG5-LIKE; APG5L; ASP; hAPG5
Summary The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]


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Customer Reviews 
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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