ATG5 Rabbit Polyclonal Antibody

SKU TA301481

Rabbit Polyclonal Antibody against ATG5

Product Data

• Application: Electron Microscopy, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
• Recommended Dilution: Radioimmunoassay, ELISA, Immunoblotting, Knockout Validated, Knockdown Validated, Electron Microscopy, Flow Cytometry, Western Blot: 1:500, Simple Western: 1:50, Immunocytochemistry/ Immunofluorescence: 1:250, Immunohistochemistry: 1:400, Immunohistochemistry-Paraffin: 1:400, Immunoprecipitation: 1:10 - 1:500, Proximity Ligation Assay
• Reactivity: Cow, Human, Mouse, Porcine, Primate, Rat, Xenopus, Zebrafish
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein. [Swiss-Prot# Q9H1Y0]
• Buffer: PBS plus 30% glycerol and 0.1% sodium azide
• Concentration: lot specific
• Purification: peptide affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: autophagy related 5
• Database Link:
  NP_004840
  Q9H1Y0
• Background: Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
• Synonyms: APG5; APG5-LIKE; APG5L; ASP; hAPG5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Regulation of autophagy, RIG-I-like receptor signaling pathway