LDHA Human Gene Knockout Kit (CRISPR)

CAT#: KN209378BN

LDHA - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: LDHA
• Locus ID: 3939
• Components:

  KN209378G1, LDHA gRNA vector 1 in pCas-Guide CRISPR vector

  KN209378G2, LDHA gRNA vector 2 in pCas-Guide CRISPR vector

  KN209378BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001135239, NM_001165414, NM_001165415, NM_001165416, NM_005566, NR_028500
• UniProt ID: P00338
• Synonyms: GSD11; HEL-S-133P; LDHM; PIG19
• Summary: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]