Dynamin 2 (DNM2) Human Gene Knockout Kit (CRISPR)

CAT#: KN208525RB

DNM2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: Dynamin 2
• Locus ID: 1785
• Components:

  KN208525G1, Dynamin 2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN208525G2, Dynamin 2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN208525RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001005360, NM_001005361, NM_001005362, NM_001190716, NM_004945
• UniProt ID: P50570
• Synonyms: CMT2M; CMTDI1; CMTDIB; DI-CMTB; DYN2; DYNII; LCCS5
• Summary: Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq, Jun 2010]