YY1 Human Gene Knockout Kit (CRISPR)

CAT#: KN207198

YY1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: YY1
• Locus ID: 7528
• Components:

  KN207198G1, YY1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAGACCATCGAGACCACAG

  KN207198G2, YY1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGAGGTGGAGACCATCCCGG

  KN207198D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CAGGGCGCGT GCGAGCCGCC CCGCCCCCGG TCCCATCGGC CCCAATCCGG GAGGAGCCCG GCGAGTGGGC GGGGCCGCGG AGGCCAGCGG ACAGATCGAT TGGCCGAGAG GAGAATCGAG AGGGCGAACG GGCGAGTGGC AGCGAGGCGG GGCGGGCTGA GGCCAGCGCG GAAGTCTCGC GAGGCCGGGC CCGAGCAGAG TGTGGCGGCG GCGGCGAGAT CTGGGCTCGG GTTGAGGAGT TGGTATTTGT GTGGAAGGAG GCGGAGGCGC AGGAGGAAGG GGGAAGCGGA GCGCCGGCCC GGAGGGCGGG AGGAGGCGCG GCCAGGGCGG GCGGTTGCGG CGAGGCGAGG CGAGGCGGGG AGCCGAGACG AGCAGCGGCC GAGCGAGCGC GGGCGCGGGC GCACCGAGGC GAGGGAGGCG GGGAAGCCCC GCCGCCGCCG CGGCGCCCGC CCCTTCCCCC GCCGCCCGCC CCCTCTCCCC CCGCCCGCTC GCCGCCTTCC TCCCTCTGCC TTCCTTCCCC ACGGCCGGCC GCCTCCTCGC CCGCCCGCCC GCAGCCGAGG AGCCGAGGCC GCCGCGGCCG TGGCGGCGGA GCCCTCAGCC AGGAGGAGGA CGACGACGAC GAGGACGGCG GCGGTGGCGA CCACGGCGGC GGGGGCGGCC ACGGGCACGC CGGCCACCAC CACCACCACC ATCACCACCA CCACCACCCG CCCATGATCG CTCTGCAGCC GCTGGTCACC GACGACCCGA CCCAGGTGCA CCACCACCAG GAGGTGATCC TGGTGCAGAC GCGCGAGGAG GTGGTGGGCG GCGACGACTC GGACGGGCTG CGCGCCGAGG ACGGCTTCGA GGATCAGATT CTCATCCCGG TGCCCGCGCC GGCCGGCGGC GACGACGACT ACATTGAACA AACGCTGGTC ACCGTGGCGG CGGCCGGCAA GAGCGGCGGC GGCGGCTCGT CGTCGTCGGG AGGCGGCCGC GTCAAGAAGG GCGGCGGCAA GAAGAGCGGC AAGAAGAGTT ACCTCAGCGG CGGGGCCGGC GCGGCGGGCG GCGGCGGCGC CGACCCGGGC AACAAGAAGT GGGAGCAGAA GCAGGTGCAG ATCAAGACCC TGGAGGGCGA GTTCTCGGTC ACCATGTGGT CCTCAGGTGA GCGCCGGCCG CGCGCCCCGG CCCCGGGATG TTTTTGTTTT GAAGGGAAGC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_003403
• UniProt ID: P25490
• Synonyms: DELTA; INO80S; NF-E1; UCRBP; YIN-YANG-1
• Summary: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]