Interleukin 34 (IL34) Human Gene Knockout Kit (CRISPR)

CAT#: KN206629

IL34 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Interleukin 34
• Locus ID: 146433
• Components:

  KN206629G1, Interleukin 34 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCTATGGTTACGGCAGATCC

  KN206629G2, Interleukin 34 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCTGGCTGCGCTGTGAGTAC

  KN206629D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CTGCCAGCTG GTGTCATCAT AACTCACTCA TGGGCCAGGG CCGTTCGGAC ATCCTGAAGT CATTTCTCAC GTGAGCCGTG TCCAGCTGCC AGGGCCGGGT GGTCCTGTGC AGGCCGTGGG TGCGGTGTGC GCTGTGGCTG ATGGCTGAGT GGCAGCGCTG CTCTTACATA ATCAGTTTCT CTCCTCCTTT CTCTTCCTAG AGCCAGATTT CACACTGAGC AGCTGCAGTC GGAGAAATCA GAGAAAGCGT CACCCAGCCC CAGATTCCGA GGGGCCTGCC AGGGACTCTC TCCTCCTGCT CCTTGGAAAG GAAGACCCCG AAAGACCCCC AAGCCACCGG CTCAGACCTG CTTCTGGGCT GCCATGGGAC TTGCGGCCAC CGCCCCCCGG CTGTCCTCCA CGCTGCCGGG CAGATAAGGG CAGCTGCTGC CCTTGGGGCA CCTGCTCACT CCCGCAGCCC AGCCACTCCT CCAGGGCCAG CCCTTCCCTG ACTGAGTGAC CACCTCTGCT GCCCCGAGGC CATGTAGGCC GTGCTTAGGC CTCTGTGGAC ACACTGCTGG GGACGGCGCC TGAGCTCTCA GGGGGACGAG GAACACCACC AACCAGGGCA TTCTTGCTGG TGAGAAGTTG CGATGCTTCC CAGCCGGACT GCAGACACCC TCTGAGATTC CCACGGCCGC CGTCTGCCCT GTCTGCCGCT CACTTCGGGC TCCGACGGGC AAGCTGGGTG GGCAGTTCTG GCTGGGTGGG CATGGTCCTC AGGGTGGTCT TAGGTCGGGG CAGGCACTGG GGGTAGGTGG CAGCATCTCA CCTTTCCAGG CTTGTGGGTG TTGGGGAGCC CCGGGCGGGC AGCAGCGGCC CAGTCCTGGG TATGGCCGGT GGAGGGGGGC AGGGGCTGGT GAAAGTGTCT AGGGACCCAA GACCCACGGC AGAAGAGCAG CCAGATGCCT GGAGAAGGCA GCCTGTTCCC CTCCAACCCT CCCTCCATAA CAGAACAGAA TTAAAAAGCA GAAAGATAAC ACTGTTGGTA AAAAGAAAAA TAAGTTGAAA TGAGGACAGA AAGGAAAAAA AGGATCTGGA AGCCTCTTAT GCCTGCTGGC TGGGAGGCCA GACCAAGATC CCCTCAGTCT CCAAGATCCA GTTCAATTGA CTCAAATTCA ACACATTTTT CCGGTGCCTC CCACGTGCCC ACTGCAGAGT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001172771, NM_001172772, NM_152456
• UniProt ID: Q6ZMJ4
• Synonyms: C16orf77; IL-34
• Summary: Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]