Interleukin 34 (IL34) (NM_152456) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Interleukin 34 |
| Synonyms | C16orf77; IL-34 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_152456 |
| ORF Size | 726 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_152456.1, NP_689669.1 |
| RefSeq Size | 1796 bp |
| RefSeq ORF | 729 bp |
| Locus ID | 146433 |
| UniProt ID | Q6ZMJ4 |
| Cytogenetics | 16q22.1 |
| MW | 27.5 kDa |
| Summary | Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 PubMed 18467591).supplied by OMIM, May 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_152456" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206629L1 | Lenti ORF clone of Human interleukin 34 (IL34), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC206629L2 | Lenti ORF clone of Human interleukin 34 (IL34), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RC206629L3 | Lenti ORF clone of Human interleukin 34 (IL34), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC206629L4 | Lenti ORF clone of Human interleukin 34 (IL34), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG206629 | IL34 (tGFP-tagged) - Human interleukin 34 (IL34), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC100981 | IL34 (untagged)-Human interleukin 34 (IL34), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice
Mathews, Branch Woods, Katano et al
Mol Neurodegener (2019) 14 (1), 12 DOI: 10.1186/s13024-019-0311-y
Mathews, Branch Woods, Katano et al
Mol Neurodegener (2019) 14 (1), 12 DOI: 10.1186/s13024-019-0311-y
AI Summary: OriGene’s pCMV6-XL4 vector containing human IL-34 cDNA was used to generate transgenic mice expressing human IL-34, enabling the study of human microglia-like cell differentiation and persistent HIV-1 infection in a humanized mouse model.
Snippet: To generate the hIL-34 expressing transgenic NOG mouse (NOG-hIL-34), a linearized DNA vector (pCMV6-XL4) containing human IL-34 cDNA (Origene Technologies, Inc., Rockville, MD, USA) under the control of a CMV promoter, was microinjected into fertilized. Cached
IKKβ and mutant huntingtin interactions regulate the expression of IL-34: implications for microglial-mediated neurodegeneration in HD
Khoshnan, Sabbaugh, Calamini et al
Hum Mol Genet (2017) 26 (21), 4267-4277 DOI: 10.1093/hmg/ddx315
Khoshnan, Sabbaugh, Calamini et al
Hum Mol Genet (2017) 26 (21), 4267-4277 DOI: 10.1093/hmg/ddx315
Cited by 33
AI Summary: In this study, OriGene’s shRNA lentiviral particles targeting IKBKB (TL312017V) and control shRNA lentiviral particles (TR30021V) were used to knock down IKKβ expression in neurons, enabling the authors to demonstrate that IKKβ is required for mutant huntingtin aggregation and subsequent IL-34 production.
Showing 1-2 of 2 papers.
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