RAMP2 Human Gene Knockout Kit (CRISPR)

CAT#: KN206531

RAMP2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: RAMP2
• Locus ID: 10266
• Components:

  KN206531G1, RAMP2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CACGATGGCCTCGCTCCGGG

  KN206531G2, RAMP2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTCTCCCTAGGACCCGAGTC

  KN206531D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CCGCTTCTCC TCCCTGGTTT TGGCTGGCGG ACTGACATCA GCTGGTATTC CTAGGGATAG GATAAGGAAC CCTGAGCTGC ATGGCTCAGC TCCAGGCGCC CCTTCCCCCA CAACCCAGGA CAGCCCCTTA GAAGCAGCGG CGTCGAGAGG CCGCTGGAGC TCCCAGCCCC CTCCCTCTTC TCCACGTCTG GGAGAGCAGC TTGCCGACCC CTCCAGACCC GAAAGAGAGA GGGGGCGACT CGAATTCGGG GAGCGATCCT GGGAGACCCT GGAGAGTCCT GGGCGCCACG CAGCCGAGCC CCCGAAATAG ATCCCGGTTC CGGGGTTCCG GGAGCTGTCC AGCCTCGCGG GGTGAGCTAC GAGAGGGTGG GCCAACGCCG GCGAGGCGGA GGGAGCCGCG GGCGCCAGGC GCGGGTGAGG CAGCGACTCC CCCTCCAGCC GCGGGCCCCG AGCGTCGCCC CCGCCCCCCA CGTGGCCGCC GCCGTCCCAG CCCCTCCGAG GAAGCGGCGC GGCTTCCTGC AGCTTGGGCT GGGGATATAG GCGCCCCCAC ACCCGGGCCC GGCTCAGCGC CGCCGCCGCT CCTCGCCTCC TTGCTGCACG GAGGGGAGAG GGGCTGGATG GCCGAGGCCG GAACGGGCCC TGGGGTGCGG GTTAGGACCG ACGTACCTAG CAGCACTGGC CCTCGGACGG TCCCTGACCC CACCTCGGGG CGGGCGCAGC ATGAGCTGCT TCCCACCCAG GGAAAGCTGG GGTGCTGGCC CCGGCCCCTC GAAGAGGGCT TAGGAGGACG GAAGCTGGCC AGAGATGAGG GGGCTTTGGG CCTGGGTGTG AGTGACAAGG AGCTGGTGCC AGCCCCTCCC TCCCCGGCAC TGAGGCGTCC GTGGGGGCTA GATTATTCCT CCTTTTCTTC CAAGGTAGCC TATTTTCGGA GGGTCTCAGT CCCCCAACCC CCCTCGCAGG CTCCACTGCC GGGGTCCCCG CTATGTTACC CTCCTCTCCC GTTTCTTCCC ACAGCTGTCC TGAATCCCCA CGAGGCCCTG GCTCAGCCTC TTCCCACCAC AGGCACACCA GGGTCAGAAG GTGGGTACCC AGGGTGTGGA AGGGTGGCCG AGGGTAAGGA CGAGAGCAAG TTCAGTGGGG AGGGTTCCTT TCCCACGAGG CCTGCCCAAC CCCAGCTGAT GCCCCACTAC ACTCCCCTCT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_005854
• UniProt ID: O60895
• Summary: The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq, Jul 2008]