Endothelin B Receptor (EDNRB) Human Gene Knockout Kit (CRISPR)

CAT#: KN204899

EDNRB - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Endothelin B Receptor
• Locus ID: 1910
• Components:

  KN204899G1, Endothelin B Receptor gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AAGTCTGTGCGGACGCGCCC

  KN204899G2, Endothelin B Receptor gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTGCGGCCTGTCGCGGATCT

  KN204899D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TCCGAATCTC CGGCGCACTC CTGGGCGCGC TGCGGGAGCT GTAGCTCAGC CAGCCAGGGA GTAGCGGCTT TCATCCGCCG GGAGGAGTCT TTCGAGTTCA ATCGCGGGGT ATAGAGGTTC CCCTGCGGGG CAAAATGCAG AGCTTGACAC AAGCCCTTGG CCTCTAGGTG CCTTAATTCC GCGGTTCCCA CGCACGCTTA ACTAAGACGT GTCTGTATTC CTCCCGTTAC GTGAAAGAGT TCGGAGCTTT GCCTGGGACC CCCATCATTC CCTCCCTGGC ACACCCCTTC CAGAACGCCC CGCCCCACTG CATATTATTT ACCCCTCCTG GCCACGCGGG GGAAGAAAAA CAGCTGAGAG GGCATCAGGA AGGAGTTTCG ACCCGCGCTG GCGAGTCATG AGCGCCAAGT TTCCCACTGG CGCGCAAACT TGAGTTACTT TTGAGCGTGG ATACTGGCGA AGAGGCTGCG GGCGGTATTA GCGTTTGCAG CGACTTGGCT CGGGCAGCTG ACCCAAGTGT CCTGTCTTCC TTCCTCTGCT TGTCTCTAGG CTCTGAAACT GCGGAGCGGC CACCGGACGC CTTCTGGAGC AGGTAGCAGC CAGAGATAAT GACGCCACCC ACTAAGACCT TATGGCCCAA GGGTTCCAAC GCCAGTCTGG CGCGGTCGTT GGCACCTGCG GAGGTGCCTA AAGGAGACAG GACGGCAGGA TCTCCGCCAC GCACCATCTC CCCTCCCCCG TGCCAAGGAC CCATCGAGAT CAAGGAGACT TTCAAATACA TCAACACGGT TGTGTCCTGC CTTGTGTTCG TGCTGGGGAT CATCGGGAAC TCCACACTTC TGAGAATTAT CTACAAGAAC AAGTGCATGC GAAACGGTCC CAATATCTTG ATCGCCAGCT TGGCTCTGGG AGACCTGCTG CACATCGTCA TTGACATCCC TATCAATGTC TACAAGGTAA AGGGCGCCTG CTGAAATCAT GGTGGGCTTG AGGAAGGGGG CCTGGTTGGA GACGGGGAGA TGAGAAAGCT TGTAGAGGGA GCTTCCCTTT AGCTCCCTCT TAAGGTTCTG CCCCTCCTAA AAGTCAGTAA TCTTTGGAAC CAAATTCCAT TTGGTTCTGT CCACTCATAA AAGTCAGTAA CCATTGGAAT AAAATTCCAT TCAGGTCTTC AGTAGACCTC TGCTAAGCTT AGGACAAGCG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000115, NM_001122659, NM_001201397, NM_003991
• UniProt ID: P24530
• Synonyms: ABCDS; ET-B; ET-BR; ETB; ETBR; ETRB; HSCR; HSCR2; WS4A
• Summary: The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]