PAH Human Gene Knockout Kit (CRISPR)

SKU KN204694RB

PAH - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Vector: RFP-BSD
• Target Symbol: PAH
• Locus ID: 5053
• Components:

  KN204694G1, PAH gRNA vector 1 in pCas-Guide CRISPR vector

  KN204694G2, PAH gRNA vector 2 in pCas-Guide CRISPR vector

  KN204694RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000277, NM_001354304
• UniProt ID: P00439
• Synonyms: PH; PKU; PKU1
• Summary: This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Aug 2017]
• Shipping: Ambient