CD147 (BSG) Human Gene Knockout Kit (CRISPR)

SKU KN203894

BSG - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Vector: GFP-puro
• Target Symbol: CD147
• Locus ID: 682
• Components:

  KN203894G1, CD147 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GATATTTGGGGGCGGCGAGT

  KN203894G2, CD147 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGGACGCGTCTCCCCAAGAA

  KN203894D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GCAGGAGAAT CGCTTGAACC CGGGAGGCGG AGGTTGCAGA GAGTCGAGAT CGCGCCGCTG CACTCCAGCC TGGGTGACAC AGCGAGACTC CAACACACAC ACACACAAAA GATATGGCGC GTCTCATTCA TTCACAAGTT CCTCTTCAAA GGTTTGGCTC GTTCAGCGCC CTCGTTCTTC GTTTCCTACT TCCAAGGCCA ACCTGACTTC CTCACCCTCT GGGCCTCCCC GTCTGAGTCT CAGTAAACAA GTTTCCCGCC GGTCCTGATC TGCAGAGATG GCACCTGCCA CCCACTCTGC ACATCCCCCT CCCGTCGCCA CGGGCTCTTC CCGCCAGTGT AGCCACATTC CTGCCCCTTT CCAGTTAGCC CTTCGCGTTC GGCTTAGTCT GCGGTCCTCT TGCATTGCGA CTCCGAGTTT AACTTCCAAC ACACACTTTC AACCTCCAAG AGACGCCCCC ACCTGTGTCG CCCCAATAGC GACTTTTCTC ACCGTGGTCG CCGCGGAACT TCAAGGGTCC TTCCTACCCG CGTTGCTGAG AGTCTGGGTT TACGCGTCAC CTCGGGCGGG ACCCGATCCT CCGCTCCTGA GGCCCCCACA TCCCGCAAAG AGAAACCAGC ACTTAGAAAA TTTCTCAGCG AGACCACTTT ACTTGTACGG AAGGGGGCTG CCTGCGTGGG ATGCAATCTC CAGAGCACAC GGGACAAAGG AAGGGAGCTT CTGTTCCTCT GTCCTTTCCC TGTTGGCTGG GGTTGGACGC CCACAATCTA AGCTGATCCC GGTTGGCTAA AACTTAAAAC TTTTTTAAAA TAGGGTAAAC GCGCCACCTG CGAGGAAAGA AGAAGGGGGT AAGGTTGATT GACAACTTTA GACCTGGAAG TTGAGTTTCT GAAGAGGAAC TTACTTGTCC CAACCAAATG GTGCACAGGA CGTTTATACT AAGAAATTAT TCATTTTCTG AGATCCAGAT TCAAGTGCCC TTTAAGTATT TTTATTTGGG CGGAGGAGGT CACTTCCTCC CACCTAATTT ATTAATACGT TCATATATAT ATATATATAT GTACATTTTT AAAGGCAGAG ATGTGCGTCT CGCCATGTTG CCGCGTCTGG TCTCGAGCTC CCGGGCTCAA GCGATCTTCC CGCCTCGACC TCCCAAGGCT CTGGGAGTAC AGACGTGAGC CACAGCGGCC GGCCCGTGTT

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001322243, NM_001728, NM_198589, NM_198590, NM_198591
• UniProt ID: P35613
• Synonyms: 5F7; CD147; EMMPRIN; OK; TCSF
• Summary: The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020]
• Shipping: Ambient