TENT2 Human Gene Knockout Kit (CRISPR)

CAT#: KN203610

PAPD4 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: TENT2
• Locus ID: 167153
• Components:

  KN203610G1, TENT2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGAATAAACAGTAGGTGACA

  KN203610G2, TENT2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GATGATTTGGAGTGAAGGGT

  KN203610D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GATTCTGATT GCTTTGTACA ATTTTTCCCT ATTCCTGTGG AACATAGCCC TCTTTTATTG GAAGTCTTTT ATATAAATTA TGTAGACTTT GAGGGGAATC TCAGAATCTT ATAAATTGTT AAGACAATAT AAAATCCATT CCTTATTTGA TCAGACCTGT AAGATCCAAA TTCTAGGAAT CAGGATGTCA TGTGATGGTT TATACCTTTG TATAAATTAG TTTGAGAATA AATGTAATAA TCTGTGGGAT TTTATTAATG TCAGGTGATA ACTTGGATTT CTAAAAGATT TGTTGATACG AGGGTAAAAG TATTAGGGTA AATTAATCAG AAAATTAGAA ATGTGCATAT ATCTTGCTGT TTGAATTATG TTAATCTTAG ATTTGGAATT TATTATTAGT TTTCTTTTGT TACCTACAGT GGCACTTAGG AGACCTATTT AACTTTGCAA ATAAAATTTG TAACTTGATA ACTAATTGTT TTAAGTTAGT GGATAATAGT TATCAAAGTG TTTAGTGTCT TTAAGCTATG ATAATTTAAT ATTGTCTTTT TAAATTATCC TAGGTAGAAG AATACATGTT CACTTCCAGT GAACAAGAGC GATGCACAAT TCAACTTTCA GAATGCAGAG TGAGTATGGT GATATTTTGG CCCATGTTGT TGGTAAATTT CATTTTCTAT TTGACATAAA CCCTTTTTGT ATGAAACTTT TGAACATGGA GAATATGTCG TCACATTTTT TTTTGATACG GATGTATTTA ATTTTTTTTC AGAGACTGGT TTTGTTTTTA AAGAAAAAAT ATGTATAAAT TACTGTTCTT TTCTTTGATT TTGTTAGCTT GTCTAGAGCT GTGTCATTAC AGCAGCTGAC ATATGGAAAT GTCAGTCCAA TACAGACCTC AGCTTCCCCA TTATTTCGAG GAAGGAAGTA AGTACTTCTT AATTATTTTA AAAGAATATT TTTGCATTTC TGAGAACTCT GTAATGATGT ATCTTGAATT AATATTGTAT GGACCCTAAG CCCTATGTTT TGTTTTCTGG GACCTGTACG ATCAGATGAG TCATGGAATT TTAACATTTT TATGCGTTTA AAAATTTTAG GTGTTCTTTT GTTGATTTGT TCCTGCTTTA TTGTATGTTT GTTTCTGCCC TACATCAGAT CTCCCCTTTT CTATATCCCA GGTTTTCTTT TTCTAGAATA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001114393, NM_001114394, NM_001297744, NM_001297745, NM_173797, NM_001349548, NM_001349549, NM_001349550, NM_001349551, NM_001349552, NM_001349553, NM_001349554
• UniProt ID: Q6PIY7
• Synonyms: GLD2; TUT2
• Summary: Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs (PubMed:15070731). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Adds a single nucleotide to the 3' end of specific miRNAs, monoadenylation stabilizes and prolongs the activity of some but not all miRNAs (PubMed:23200856).[UniProtKB/Swiss-Prot Function]