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TENT2 (NM_001114394) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC225825
PAPD4 (Myc-DDK-tagged)-Human PAP associated domain containing 4 (PAPD4), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TENT2 or NM_001114394"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TENT2
Synonyms APD4; GLD2; PAPD4; TUT2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC225825 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCCAAACTCAATTTTGGGTCGCCCACCCTTCACTCCAAATCATCAACAACATAATAACTTCTTTA
CCCTGTCACCTACTGTTTATTCACACCAGCAGCTTATAGATGCACAATTCAACTTTCAGAATGCAGACTT
GTCTAGAGCTGTGTCATTACAGCAGCTGACATATGGAAATGTCAGTCCAATACAGACCTCAGCTTCCCCA
TTATTTCGAGGAAGGAAGAGATTAAGCGATGAAAAAAACCTTCCTCTTGACGGTAAACGGCAACGTTTCC
ATTCACCCCACCAAGAGCCAACTGTAGTTAACCAGATAGTGCCTTTATCAGGTGAACGAAGATACTCAAT
GCCACCATTGTTTCATACACATTATGTACCAGATATAGTCAGATGTGTTCCACCTTTTCGAGAAATTGCA
TTTTTAGAACCTAGAGAAATCACACTGCCTGAGGCCAAAGATAAGTTGAGTCAGCAGATACTGGAGTTAT
TTGAAACATGTCAGCAGCAAATAAGTGATTTAAAGAAGAAAGAACTCTGTCGAACACAGCTGCAGAGAGA
AATTCAGCTGTTATTTCCACAAAGCAGACTTTTTTTGGTTGGGTCCTCTTTAAATGGATTTGGTACCCGG
AGCAGTGATGGTGATTTATGCCTAGTTGTTAAGGAAGAACCATGTTTTTTTCAGGTAAATCAGAAGACTG
AAGCACGGCATATACTCACCTTAGTCCATAAACACTTCTGTACTAGACTTTCGGGCTACATTGAGAGACC
TCAGCTGATTCGAGCAAAAGTGCCAATTGTGAAGTTCAGGGATAAAGTCAGTTGTGTGGAGTTTGACTTG
AATGTAAACAATATTGTTGGAATAAGAAACACATTCCTTCTCAGAACTTATGCATACCTTGAAAATCGAG
TTCGTCCGTTAGTGCTGGTGATTAAGAAGTGGGCAAGTCACCATCAGATAAATGATGCCAGTCGTGGTAC
TTTAAGCAGCTATAGTCTTGTATTGATGGTTTTGCACTATTTACAAACCCTACCTGAACCCATCCTTCCA
TCCCTCCAAAAAATTTACCCAGAGTCTTTTAGTCCTGCTATACAGCTGCACCTTGTACATCAAGCTCCAT
GTAATGTTCCTCCTTACCTCTCAAAGAATGAATCAAACCTTGGGGACCTCTTACTGGGCTTTCTTAAATA
TTATGCTACAGAATTTGACTGGAATAGTCAAATGATTTCAGTTCGTGAAGCCAAAGCCATTCCAAGGCCT
GATGGTATTGAATGGAGAAATAAATACATCTGTGTAGAAGAACCTTTTGATGGAACAAATACAGCCAGAG
CAGTGCACGAAAAGCAGAAATTTGATATGATCAAGGATCAATTTTTAAAGTCATGGCACAGATTGAAAAA
CAAGAGAGATTTGAACAGTATACTACCTGTAAGAGCTGCTGTCCTGAAAAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC225825 protein sequence
Red=Cloning site Green=Tags(s)

MFPNSILGRPPFTPNHQQHNNFFTLSPTVYSHQQLIDAQFNFQNADLSRAVSLQQLTYGNVSPIQTSASP
LFRGRKRLSDEKNLPLDGKRQRFHSPHQEPTVVNQIVPLSGERRYSMPPLFHTHYVPDIVRCVPPFREIA
FLEPREITLPEAKDKLSQQILELFETCQQQISDLKKKELCRTQLQREIQLLFPQSRLFLVGSSLNGFGTR
SSDGDLCLVVKEEPCFFQVNQKTEARHILTLVHKHFCTRLSGYIERPQLIRAKVPIVKFRDKVSCVEFDL
NVNNIVGIRNTFLLRTYAYLENRVRPLVLVIKKWASHHQINDASRGTLSSYSLVLMVLHYLQTLPEPILP
SLQKIYPESFSPAIQLHLVHQAPCNVPPYLSKNESNLGDLLLGFLKYYATEFDWNSQMISVREAKAIPRP
DGIEWRNKYICVEEPFDGTNTARAVHEKQKFDMIKDQFLKSWHRLKNKRDLNSILPVRAAVLKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001114394
ORF Size 1452 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001114394.3
RefSeq Size 3523 bp
RefSeq ORF 1455 bp
Locus ID 167153
UniProt ID Q6PIY7
Cytogenetics 5q14.1
MW 56 kDa
Summary Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs (PubMed:15070731). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Adds a single nucleotide to the 3' end of specific miRNAs, monoadenylation stabilizes and prolongs the activity of some but not all miRNAs (PubMed:23200856).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001114394" in other vectors (4)
SKU Description Size Price
RC225825L3 Lenti ORF clone of Human PAP associated domain containing 4 (PAPD4), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC225825L4 Lenti ORF clone of Human PAP associated domain containing 4 (PAPD4), transcript variant 1, mGFP tagged 10 ug
$757.00
RG225825 PAPD4 (tGFP-tagged) - Human PAP associated domain containing 4 (PAPD4), transcript variant 1 10 ug
$489.00 $657.00
SC318908 PAPD4 (untagged)-Human PAP associated domain containing 4 (PAPD4), transcript variant 1 10 ug
$503.00

Plasmid Map for RC225825

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC225825
Circular map for RC225825

file-alt Citations

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