COX11 Human Gene Knockout Kit (CRISPR)

SKU KN203238

COX11 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Vector: GFP-puro
• Target Symbol: COX11
• Locus ID: 1353
• Components:

  KN203238G1, COX11 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCCTGGTTGGAGACCCAGGG

  KN203238G2, COX11 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ATCCAGCGCCAGCCACAGAA

  KN203238D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AAACTTCAGC GTGACAGCCT CCCTCCTGCT CCAGCTGTGG TGCTTTCCAC TGGGCAAGTT GTAAGGGGCA TCAGAGCAGG GGAAGCCGAC TGCCCACGGG CTGAGAAAAT GCAGGTGTCG TGGGTGCCCA TGAGGGCTGC GGTTAACACA GCAAGGCTTC CTGAGAGAGG TGATACTTGA ACTAAGTCCA GGGCAGTGAG CACAGGAGCC AGCGGCCAAG GGACCCCAGG AGAAAAGACC GCGGGAGAGC GTCGCCGGGG CTGCCGGGTC GAGACAAAGG CTGGTGGAGT TTAAATAAAG ATCACAGGCG CAACCCCAAG TGATGGCAAG CTTGCACGCG GACGGGCGAA ACGCATTTTC CTAAATGTTA AGAGACGTTC TGGCGAGAAG CGAAGTGGGA GTAACAGTCA CAAAGCAAAA CGCAAACTTA CCCGTAATCT GGCAACCCGG CCTCCGTAGC CCGACTGCAA GCGCTGCCAC CTGAGGAGCC TGGTAGCCAA GGCAGCGGCA GGCTCAACCC AGATCTCGCG AGACGGAGCA CGCCTCGCGA GATTTGACCT CTCGTCCCTG AGAGGCGGGT GGGTGTTAGT TCAGAGGGTT CGTTTCTTAG GCCAGAGTGG AGTGGGACAG GAGGTGCCGA GAGAGGACTG AGGTGGCTTG GGACATGGAA GCGCTGCAGC CTTCGAGCCC GGCATCCAGC ATTGCAGCCG CCGCGGCGGC CTAAGAGCTC GAACCCTTTC ACACGCGCGC AGGAGGAGGA GCGGCGGCGG CAGAACAAGA CGACCCTCAC TTACGTGGCC GCTGTCGCCG TGGGCATGCT GGGGGCGTCC TACGCTGCCG TACCCCTTTA TCGGCTCTAT TGCCAGGTAG GGGCCGGCGC CGCCCGCAGG GTGGCGCAGA CGCGAGAGGT GGGGGTGCAA GACAAATCCC GGGACAAGCC CTCGCTCTGC GGGTCGTAGG TGGAAGAGGT TTCCGATATT ATCTACCTAA GAGGAGAGAG GTTCAAAACA GTCACTTAAC ACCCAAAGTC ACCAAAGAAA TTGGTGACAA AACTGCAAGT AGCATCCAGT TCTTAGTGAT TGTGCTTATA CCATGCTGCA GAGTGAAACC CCTGATCTAT GAACTGCACC TCGCCAAAAA AAAAAAAAAA GGTCCGCAGC CTCTAACCGG GTATCTAGCA GTCTGCCTTA CCCTCCTGCG

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001162861, NM_001162862, NM_001321518, NM_004375, NR_027941, NR_027942, NR_135677
• UniProt ID: Q9Y6N1
• Synonyms: COX11P
• Summary: Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene has been found on chromosome 6. [provided by RefSeq, Jun 2009]
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