UFM1 Human Gene Knockout Kit (CRISPR)

CAT#: KN202665

UFM1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: UFM1
• Locus ID: 51569
• Components:

  KN202665G1, UFM1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTTCGACCTGAGGAAAAGAG

  KN202665G2, UFM1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AAAAGAGCGGGAGAGAGTCA

  KN202665D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GAGAAACTAT GAGAAATGTA AAGGATATAA CAGGATTGGT TTGCTGGAAA GACGGAAGAA AACTAATGAT AAAGCAGTGT TACAATTCCT GCTTCTGGAG CACCAATTAA GCTTTTCCGC GCTCAGGTTG CCTTAGAAAC TTTAGAGCGC GTCCGCCCCA GCCCCGCCCC CGGCCTCGCC CCGAGCTTCG CCCCAAGTCC ATCTCGCTCT TCATCCGACT ACCCCATACC CTTACTTCCG CCCCAACCTG CCAACTGGAA CCTAGGGTCC TACGCTCTAT GCCCACGCTT ATTCTGCCTG ACTAAAAATA TGGTAGCAAA TTACCAAAGT TAGAAAGATA TCTTATAAAT ATAATCCATA CTTTTTTCTT AGATATATAA ACGCAAAAGC ACAAGATATT TGTAATTTAA AAGCGCCATC TAGCATGAGC TCTTAGGGCC AATTTTCAGG CAGTGCGTTG GATAGCAACA ACTTCGGAGG TCCCCAGATT GCAGAGGGAG ACGTGGACGT GAGTGGAGCG GGGCGGTCCC CAGCACACTA GAGGAAGTCG TGCTACCCCC GCGGAGTTGT CGTGTGTTCT GGATTCATTC CGGCACCACC ACGCTGACGT CGGACCCACG GCTGCCGTAC AAAGTGTGAG TAGCTCGGCC GAGATGGGCC TTTTGGGGCC GGACAAGACG GGGCTGGGTT GGGGATGATC CGAGCTTTTC CAACAACTAC CCCACGCAGT CTTCATCTCT TCACTTCATC TACTTTCCTG GCTCGCGCCC TTCCAGGAGC CTTTCCCACC GGAGCCTGCG AGGAGAGGTC CGTACTTGCT CGCTAAGTGT CAGCTTGGCA GCTTGGCCCC GTCACGAGGG TGTGGGTGTG TTTCTGATTA GTGACCTCCC CTCGGAATTC ATTTGGTGGG CAGGTGGCGC GGGAGGACAG GTGGACCAGG TTCTGGTAAT TTGTTGGGAT TCTTAAACAG TCCCCATCTG CTGGATATTA GAAAAGCGTT TCACCGCTTT GGCCAGCTGG TTGAGGAAGT TAAGGAAGAC ATTCCATCTT GATAGTCAAG ACTTTGAAAT TACTTGAACA TTTGTTATTC CGCGGCTTCT GAGTTTCGTT GTGTATGAGT AACTCAGCAG TCGGGCATCA CAAAGTAATG AGACTCAGAT CACACCCTTC CTATGCTGGG ATTTAATAGA GTATTTGGTA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001286703, NM_001286704, NM_001286705, NM_001286706, NM_016617, NR_104584, NR_104585
• UniProt ID: P61960
• Synonyms: BM-002; C13orf20
• Summary: UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Dec 2008]