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XBP1 Human Gene Knockout Kit (CRISPR)

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SKU
KN201959RB
XBP1 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

Frequently Bought Together (3)

RC201959 XBP1 (Myc-DDK-tagged)-Human X-box binding protein 1 (XBP1), transcript variant 1
10 ug
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TT300007S A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
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Other products for "XBP1 or NM_001079539"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Vector RFP-BSD
Target Symbol XBP1
Locus ID 7494
Components

KN201959G1, XBP1 gRNA vector 1 in pCas-Guide CRISPR vector

KN201959G2, XBP1 gRNA vector 2 in pCas-Guide CRISPR vector

KN201959RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

Homologous arm and RFP-BSD sequences:

pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

GACTTCTATT CCGCAGAATT TCTTTCCAGG CTTTTTTCTT TTTCTTTTTT TGAGACGGAG TCTCGCTCTG TCGCCCAGGC CGGGGTGCAG TGGCGCGATC TCGGCTCACT GAAACCTCTG TCCAGTCTTT TCGAACCCAA GGCCCAACTG CGCTCTATCT CGACTTTCGG CTCCACTCGG ATCCCGAAGT GGCGCACGAG ATAAAATGTT GTCAGGCTGA GGTAATTCTC TGTTAGTCCC GGTAAAAATT CGTCAGTCTG GAAAGCTCTC GGTTTGGAAT TAAATTCTGT CACTCCGGAT GGAAATAAGT CCGCTTAAGG GGGGAAAATC CGTTTGTGGA GGACACGCTC CCGCACGTAA CCCCCCGCGG AAAATGACCC CAAGTACCTT TGGCCAGGGA TTGCCGCTGC CACGCCGGAC TCCATAGCCA CGGTCCTGAA ACGCCCCGCC GGGCAGGCCG GACCAATGGA CGCCGAGCTC GGCCGTGCGT CACGCGACGC TGGCCAATCG CGGAGGGCCA CGACCGTAGA AAGGCCGGGC GCGGCGAGGC TGGGCGCTGG GCGGCTGCGG CGCGCGGTGC GCGGTGCGTA GTCTGGAGCT CTCATGGTGC CAGCCCAGAG AGGGGCCAGC CCGGAGGCAG CGAGCGGGGG GCTGCCCCAG GCGCGCAAGC GACAGCGCCT CACGCACCTG AGCCCCGAGG AGAAGGCGCT GAGGAGGTGG GCGAGGGGCC GGGGTCTGGG GCCAGATCTG AAGCCGGGAC TAGGGACAGG GGCAGGGGCA GGGGCTGGGA GCGGGGACCC AGCACTGGCC GCCCCGCAGG GCTCCGTCGC CTTTGGCCTG GCGGGTCGGT GCCAGCGTGG CGCGGGGCGG GGCAGGAAGC CCGGACTGAC CGGATCCGCC ACGCTGGGAA CCTAGGGCGG CCCAGGGCTC TTTTCTGTAC TTTTTAACTC TCTCGTTAGA GATGACCAGA GCTGGGGATG CGGGCACCTG TCTTCCAGGC CCTCTTGCTG TGTGGCCGCA GACTGGTGGT TCAGCCTCTT AACTCGGACA TGAGGTCGAA TAATCTGTTT TGGTTTACTG CTATTTCTGG AGAGGCGCGG AGCTGAAATA ACAGAGCTGT TGAAAGGGCT GGGAATTCTG CGAGGCTCAC TGGTCTAGCT CAGTATCTGC GTTCTTAAAA TGGAACCTAC TTCATGAGGT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001079539, NM_005080
UniProt ID P17861
Synonyms TREB-5; TREB5; XBP-1; XBP2
Summary This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. provided by RefSeq, Jul 2008
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Documents
Resources
Other Versions
SKU Description Size Price
GA105164 XBP1 CRISPRa kit - CRISPR gene activation of human X-box binding protein 1 1 kit
$1,657.00
KN201959 XBP1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN201959BN XBP1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN201959LP XBP1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN401959 XBP1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

ATF4 and CHOP coordinate endoplasmic reticulum stress-responsive gene programs through enhancer activation and chromatin looping
Joo, Kim, Kim et al
Nucleic Acids Res (2026) 54 (12) DOI: 10.1093/nar/gkag641
Cited by 1
AI Summary: OriGene knockout donor plasmids (KN201959LP and KN201959RB) were used as vectors to subclone HDR donor templates for generating knockout cell lines in this study investigating ATF4 and CHOP roles in ER stress responses.
Snippet: HDR donor templates containing ∼600 bp homology arms were constructed and subcloned into knockout donor plasmids (OriGene, KN201959LP, and KN201959RB).;- HDR donor templates containing ∼600 bp homology arms were constructed and subcloned into knockout donor plasmids (OriGene, KN201959LP, and KN201959RB).;t cell lines, homology-directed repair (HDR)-mediated genome editing was performed. HDR donor templates containing ∼600 bp homology arms were constructed and subcloned into knockout donor plasmids (OriGene, KN201959LP, and KN201959RB). … For generation;- HDR donor templates containing ∼600 bp homology arms were constructed and subcloned into knockout donor plasmids (OriGene, KN201959LP, and KN201959RB)
Showing 1-1 of 1 papers.
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