SMAD4 Human Gene Knockout Kit (CRISPR)

CAT#: KN201828RB

SMAD4 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: SMAD4
• Locus ID: 4089
• Components:

  KN201828G1, SMAD4 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TTGATGTGCCATAGACAAGG

  KN201828G2, SMAD4 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ACTATGCACAATGCTCAGAC

  KN201828RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  Homologous arm and RFP-BSD sequences:

  pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

  TTTTTTAATT GGTCTCTTTT AACAAAGGTG AGTGTTAACT GCTAGGTAGA ATTTCATACC AGGCGATGGT AAATTTTTCA TAAATATTAG TAATAGACTT TTAGGTTTAA CTAGTGCTGA TCCTCATAAG TAATCCTTTA AAAAAGTGCT GGTAAGATTT TCCTTTAGGG TGACTAGTAA AGTCAGTAAG ACTTGATTGA GCATGCCACT ATCATAATGA GAAGACCTAA CTTTAATATT TTTGGAATGT CAAAAACAGT GACATTTTAA TTAAGTAATT TTATTTTTAA AAATTATTTT TAATGGCTAA TATTTTGTAA GAATTTTAAG TAATTTTCAA CTCTGAGCAT CAAATTTTAA TTATGTGCAT TGAATCTCTG ACTTAACCAG AGCAATTTCA TCTTTTCCCA AGTAGTCAGA TCTACTTCGT AAAATGTGTT CTGATGTGTG TCTTTTTTTT TTTTCTTTTT TAGGTTATCC TGAATACATG TCTAACAATT TTCCTTGCAA CGTTAGCTGT TGTTTTTCAC TGTTTCCAAA GGATCAAAAT TGCTTCAGAA ATTGGAGACA TATTTGATTT AAAAGGAAAA ACTTGAACAA ATTTGCAAAA AGAGCAATTG AAAGTTTGGT AAAGAAGCTG AAGGAGAAAA AAGATGAATT GGATTCTTTA ATAACAGCTA TAACTACAAA TGGAGCTCAT CCTAGTAAAT GTGTTACCAT ACAGAGAACA TTGGATGGGA GGCTTCAGGT TAGTCTTATA AGAGTTTTTC TATACCCTCT ATGGTGGCAG ATTTAAAAAC TTGCTACGTT TCCTTTCAAG CTACTACAGG GTAATTTAAT TTGTGCTCCA TCTCTTCAGA TACTGTGCAT CCTGTACAAA TATGCATTAT GGGAATTTCT GGAAGAATAT GCAAAAGATA AAATCTTAAA GTTTTTTAAT GTTCTACTCA GAAAAATGTT CAATGGAGAA AATTTGGAAA ATAAAAATAA AAAGCAGAAA ACCCACACTT CGCAAGAACT ACCTTTAGCA CTTTGGTATT CATTTTTGTA GCCTGTTGTT TTCTTTTTTT TTTTTTTCCT AGACGGAGTC TTGCTCTGTC ACCCAGGCTG GAGTACAGTG GCGTGATCTT GGCCCACTGC AACCTCCACC TCTTGGGTTC AAGCAATCCT CCTGCCTCAG CCTCCTAAGT AGCTGGGACT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_005359
• UniProt ID: Q13485
• Synonyms: DPC4; JIP; MADH4; MYHRS
• Summary: This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Aug 2017]