SMAD4 (NM_005359) Human Tagged ORF Clone
$514.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SMAD4 |
| Synonyms | DPC4; JIP; MADH4; MYHRS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005359 |
| ORF Size | 1656 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005359.6 |
| RefSeq Size | 3220 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 4089 |
| UniProt ID | Q13485, A0A024R274 |
| Cytogenetics | 18q21.2 |
| Domains | DWA, DWB, MH1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway |
| MW | 60.3 kDa |
| Summary | This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005359" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201828L1 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), Myc-DDK-tagged | 10 ug |
$814.00
|
|
| RC201828L2 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), mGFP tagged | 10 ug |
$814.00
|
|
| RC201828L3 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), Myc-DDK-tagged | 10 ug |
$814.00
|
|
| RC201828L4 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), mGFP tagged | 10 ug |
$814.00
|
|
| RG201828 | SMAD4 (tGFP-tagged) - Human SMAD family member 4 (SMAD4) | 10 ug |
$489.00
$714.00
|
|
| SC116771 | SMAD4 (untagged)-Human SMAD family member 4 (SMAD4) | 10 ug |
$515.00
|
|
Citations
Down-Regulation of MicroRNA-210 Confers Sensitivity towards 1'S-1'-Acetoxychavicol Acetate (ACA) in Cervical Cancer Cells by Targeting SMAD4
Phuah, Azmi, Awang et al
Mol. Cells (2017) 40 (4), 291-298 DOI: 10.14348/molcells.2017.2285
Phuah, Azmi, Awang et al
Mol. Cells (2017) 40 (4), 291-298 DOI: 10.14348/molcells.2017.2285
AI Summary: OriGene's pCMV6-SMAD4 plasmid was used to overexpress SMAD4 in cervical cancer cells, enabling the authors to demonstrate that increased SMAD4 enhanced the anti-cancer effects of ACA when miR-210 was down-regulated.
Snippet: Cells were plated at density of 1.5 × 105 cells and allowed to attach overnight. Cells were then transfected with 50 ng of pCMV6-SMAD4 (Origene Technologies, USA) complexed with DharmaFECT 1 Transfection Reagent (Thermo Fisher Scientific, USA) for 12 h according to manufacturer’s protocol
M10, a caspase cleavage product of the hepatocyte growth factor receptor, interacts with Smad2 and demonstrates antifibrotic properties in vitro and in vivo
Atanelishvili, Shirai, Akter et al
Transl Res (2015)
Atanelishvili, Shirai, Akter et al
Transl Res (2015)
Cited by 11
AI Summary: OriGene products, including recombinant human Smad2 (NM_005901) and Smad4 (NM_005359) with C-terminal MYC/DDK tags, as well as the anti-DDK antibody, were used in this study to investigate the interaction between the M10 peptide and Smad proteins, contributing to the demonstration of M10's antifibrotic properties.
A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytes
Doss, Corcoran, Jima et al
BMC Genomics (2015) 16 (1), 952 DOI: 10.1186/s12864-015-2156-2
Doss, Corcoran, Jima et al
BMC Genomics (2015) 16 (1), 952 DOI: 10.1186/s12864-015-2156-2
Cited by 130
AI Summary: OriGene contributed to this study by providing the SMAD4 cDNA overexpression construct, which was used to investigate the regulatory effects of miR-4732-3p on SMAD4 in erythroid cells.
Snippet: For luciferase reporter constructs, a portion of the 3' untranslated region (UTR) of SMAD2 and SMAD4 flanking the predicted miR-4732-3p binding site was amplified and cloned into the XhoI and NotI sites downstream of Renilla luciferase in the psiCheck-2 vector (Promega). …The SMAD4 cDNA overexpression construct was obtained from Origene, and the SMAD2 cDNA (HsCD00001922) overexpression construct [65] was obtained from DNASU
TAZ promotes epithelial to mesenchymal transition via the upregulation of connective tissue growth factor expression in neuroblastoma cells
Wang, Xu, An et al
Mol Med Rep (2014)
Wang, Xu, An et al
Mol Med Rep (2014)
Cited by 45
Showing 1-5 of 5 papers.
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