SMAD4 (NM_005359) Human Untagged Clone
$515.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | SMAD4 |
| Synonyms | DPC4; JIP; MADH4; MYHRS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_005359 |
| Insert Size | 3570 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005359.3, NP_005350.1 |
| RefSeq Size | 3220 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 4089 |
| UniProt ID | Q13485 |
| Cytogenetics | 18q21.2 |
| Domains | DWA, DWB, MH1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway |
| Summary | This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005359" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201828 | SMAD4 (Myc-DDK-tagged)-Human SMAD family member 4 (SMAD4) | 10 ug |
$514.00
|
|
| RC201828L1 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), Myc-DDK-tagged | 10 ug |
$814.00
|
|
| RC201828L2 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), mGFP tagged | 10 ug |
$814.00
|
|
| RC201828L3 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), Myc-DDK-tagged | 10 ug |
$814.00
|
|
| RC201828L4 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), mGFP tagged | 10 ug |
$814.00
|
|
| RG201828 | SMAD4 (tGFP-tagged) - Human SMAD family member 4 (SMAD4) | 10 ug |
$489.00
$714.00
|
|
Citations
TGF-β-Induced PAUF Plays a Pivotal Role in the Migration and Invasion of Human Pancreatic Ductal Adenocarcinoma Cell Line Panc-1
Lee, Ham, Lee et al
IJMS (2024) 25 (21), 11420 DOI: 10.3390/ijms252111420
Lee, Ham, Lee et al
IJMS (2024) 25 (21), 11420 DOI: 10.3390/ijms252111420
Cited by 4
AI Summary: OriGene products pCMV6-XL5, pCMV-Smad4, pLenti-control, and pLenti-PAUF vectors were used in this study to manipulate gene expression and investigate the roles of PAUF and Smad4 in TGF-β-induced migration and invasion of pancreatic cancer cells.
Snippet: siRNAs targeting human Smad2/3 (cat# sc-37238), Smad4 (cat# sc-29484), and ZG16B/PAUF (cat# sc-93479) were purchased from Santa Cruz Biotechnology, and pCMV6-XL5 (cat# PCMV6XL5), pCMV-Smad4 (cat# SC116771), pLenti-control (cat# PS100092), and pLenti-PAUF (cat# RC202244L3) vectors were obtained from OriGene Technologies, Inc. (Rockville, MD, USA)
Smad3 and NFAT cooperate to induce Foxp3 expression through its enhancer
Tone, Furuuchi, Kojima et al
Nat Immunol (2008) 9 (2), 194-202 DOI: 10.1038/ni1549
Tone, Furuuchi, Kojima et al
Nat Immunol (2008) 9 (2), 194-202 DOI: 10.1038/ni1549
Cited by 808
Showing 1-2 of 2 papers.
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