GATA3 Human Gene Knockout Kit (CRISPR)

CAT#: KN201618

GATA3 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: GATA3
• Locus ID: 2625
• Components:

  KN201618G1, GATA3 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGACGGCGGACCAGCCGCGC

  KN201618G2, GATA3 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ACCACCCCGCCGTGCTCAAC

  KN201618D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CCAAGTCGTA ACAGTCAGCC CTGGGACTTG CCCTCCAAGT TGCTCAGCCA GCCCCGGCTC CCGCGAGCCG GGCTGCAGGG ACGTCCCCGA GAGCCCTGCG GGCTCCGCGG CCGTGTCCCC GCGCTCCCGT GCGGGTCTCG GGTGCGCTGG GCGGGCGGGC GGCGCGAGGG GAGGTTGTGC CACTCCAGCA ACTCAGGGGC TCATCCAGGT CTCCCATTCT CTCCCTTGCA GGTGACCCGA GGAGGGACTC CGCCTCCGAG CGGCTGAGGA CCCCGGTGCA GAGGAGCCTG GCTCGCAGAA TTGCAGAGTC GTCGCCCCTT TTTACAACCT GGTCCCGTTT TATTCTGCCG TACCCAGTTT TTGGATTTTT GTCTTCCCCT TCTTCTCTTT GCTAAACGAC CCCTCCAAGA TAATTTTTAA AAAACCTTCT CCTTTGCTCA CCTTTGCTTC CCAGCCTTCC CATCCCCCCA CCGAAAGCAA ATCATTCAAC GACCCCCGAC CCTCCGACGG CAGGAGCCCC CCGACCTCCC AGGCGGACCG CCCTCCCTCC CCGCGCGCGG GTTCCGGGCC CGGCGAGAGG GCGCGAGCAC AGCCGAGGCC CCTACATGGA CGCGGCGCAG TACCCGCTGC CGGAGGAGGT GGATGTGCTT TTTAACATCG ACGGTCAAGG CAACCACGTC CCGCCCTACT ACGGAAACTC GGTCAGGGCC ACGGTGCAGA GGTACCCTCC GACCCACCAC GGTGAGTGCG CCCGGGGTGC CGGGGCTCCC GCCGGCCGCT TCAGCCGTCC CGGCTCGGGG AGGTCGGGAG GGACCTGAGG GCGGGGAGAG GTCAAGCGAA AGCCCCCATC TGCCGTTCCT GGTTCATTTA CAAAAAAATT GGGGCCCGGA AATGGGCGAG GAAGGCCTCT GGCTCGTCGC GAGAGTGTGT TTTGAAAGAG TCGCAGCAGG CGCCTCTCCC GGCTGGTGGC CCTGGGGCCT GGCGCTCACC TGGCGGGCGC CAGGCCGCAG CCCTCCGTCT CTGCGCGGCT GCAGGTTTTG GGGTGGGGGG TCTCGGGATG TCCCCAAGCG CGGGGTGCCC TGGCTCTGCC TCCGCGGGTC CGGGCTCTCT GGGCCCGGTA GCCGGCGCCG GACAAGCACG GCTGGAACCC GGCTCTTCCA AAAAACCTGG CGTTCCCTGT TACCCGCTAG CTCTTTCTAG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001002295, NM_002051
• UniProt ID: P23771
• Synonyms: HDR; HDRS
• Summary: This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]