CPT1A Human Gene Knockout Kit (CRISPR)

CAT#: KN200485RB

CPT1A - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: CPT1A
• Locus ID: 1374
• Components:

  KN200485G1, CPT1A gRNA vector 1 in pCas-Guide CRISPR vector

  KN200485G2, CPT1A gRNA vector 2 in pCas-Guide CRISPR vector

  KN200485RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  Homologous arm and RFP-BSD sequences:

  pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

  TTTAATTGGA GTTGTCAAAC ATACACAAGA GTGAAGAGGA CTGATTAAAG ATCCCCACCA TTCCCTTCCT TGTTTCAACA ATTTTGCCAA TTTTGTTTGA TATAATCCTT TTTTTTTTTT GGCTGGAGTA TTTTACAGCA AATTCTCGAC ATGGGGTGAG GTCTCCCATG AAATCTTCAG GGTTAAGCAG CCCTTCTGAC ACAGGGGCGG CAAGAGGAGC AGTTTTGGAA CCGGGGTCTC GGGGCATTCT AGACAGTAGC AGGCTGGGGA CAGGACGTCA TGGTGGCTCA GTGACGTCCT TCACCAGCTA AACTAGTGCC TGGGCTGATT ATCATGGCCC GGGGCGTCGA CTCTTGGGGA AGCTTTCAGG GTGGGTTTTG GCTCGGAAGT AGTGGAAGGG AGGAAACTGG AATCACTGCA GGCTGTAAAC TCTGGGGAGA GTCCCAGCTA GTGGGGTGTG GTTTCTCAGT GCACGCACGC TGTCCACACC CTCTTCTCAC TGCCTGCTGT CCCAGCAGCC CTGCTGCCAG GGGCCATGAA GCGTCATTGC CCTGTGGGTT GTGGGAGGAC ACCGCTCACG GCCCGGGTGT CTGTCTTTAG TGACAACGAT GTACGCCAAG ATCGACCCCT CGTTAGGAAT AATTGCAAAA ATCAATCGGA CTCTGGAAAC GGCGTAAGTA ATCCTGTGAA ATCTCCAATT GTCTGGGAAG TTACCGATTT TATTGCTGTT TTTATGTCTC TTCAAATGTG ATGATTGTCA CTGTTCTGGA TTCCATATGA AGCTCCTGAT GCTGTTCTCA CGTCAGCTTT CTTCCTGTCT CCTGGGTGCT GTTAAGGTCC CCCTAGGAGT TTGCGGTCCC CTGCGGCCTT TCCTCTGTAG ACCAGGGTGA GAAGAGTGGT AGTGGCCCTG GGGCACCCCT TGGAAGGTGT CAGGGCCCGA GGGTTTGGTC AATAATGACA GTGCCCTTAG CCTGGCCAAT AGGGCTCTGA AATGGAATTT CTGCTCAAAG GGCTGATTCC CCATGTAGTC ATTTCTTTGC ACTTGGAGTC TGTTCATTCC AGAGTTCTGT GACCCTTGAA CTGACTGCTG TGGGGTCCTC TGCAGGGACC TCACCCACTT AACTCTGTCA GAATGGTTTA GAAATCTCCA GGGACCCCCC CGTCCCTCCT ACCTCTAGGG TTTTGGATTT TCCTGGGGCT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001031847, NM_001876
• UniProt ID: P50416
• Synonyms: CPT1; CPT1-L; L-CPT1
• Summary: The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]