CPT1A (NM_001031847) Human Tagged ORF Clone
SKU
RC200485
CPT1A (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2
$1,038.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CPT1A |
| Synonyms | CPT1; CPT1-L; L-CPT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001031847 |
| ORF Size | 2268 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001031847.2 |
| RefSeq Size | 2671 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 1374 |
| UniProt ID | P50416 |
| Cytogenetics | 11q13.3 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway |
| MW | 86.2 kDa |
| Summary | The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001031847" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200485L1 | Lenti ORF clone of Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,338.00
|
|
| RC200485L2 | Lenti ORF clone of Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged | 10 ug |
$1,338.00
|
|
| RC200485L3 | Lenti ORF clone of Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,338.00
|
|
| RC200485L4 | Lenti ORF clone of Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged | 10 ug |
$1,338.00
|
|
| RG200485 | CPT1A (tGFP-tagged) - Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2 | 10 ug |
$1,238.00
|
|
| SC321568 | CPT1A (untagged)-Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2 | 10 ug |
$693.00
|
|
Citations
Fatty acid oxidation is a druggable gateway regulating cellular plasticity for driving metastasis in breast cancer
Loo, Toh, Xie et al
Sci Adv (2021) 7 (41), eabh2443 DOI: 10.1126/sciadv.abh2443
Loo, Toh, Xie et al
Sci Adv (2021) 7 (41), eabh2443 DOI: 10.1126/sciadv.abh2443
Cited by 102
AI Summary: In the paper "Fatty acid oxidation is a druggable gateway regulating cellular plasticity for driving metastasis in breast cancer," OriGene products such as CD36 cDNA clone (Cat# RC223307) were used to investigate the role of fatty acid transport in breast cancer cell plasticity and metastasis.
Snippet: on. Fatty acid transporter, CD36, has been reported to contribute to tumor initiation (26), and a myriad of studies have expounded on the role of lipogenesis via fatty acid synthase to be vital to tumorigenesis (27, 28). Palmitate channeled to an alternative fatty acid desaturation pathway involving fatty acid desaturase 2 promotes tumor plasticity (29). Nonetheless, the mechanistic manner by whic
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
