Human POLR2J2 activation kit by CRISPRa

SKU GA116727

POLR2J2 CRISPRa kit - CRISPR gene activation of human RNA polymerase II subunit J2

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: POLR2J2
• Locus ID: 246721
• Components:

  GA116727G1, POLR2J2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA116727G2, POLR2J2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA116727G3, POLR2J2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032958, NM_032959, NM_145325
• UniProt ID: Q9GZM3
• Synonyms: DNA directed RNA polymerase II polypeptide J-related; HRPB11B; MGC54043; MGC105050; polymerase (RNA) II (DNA directed) polypeptide J2; RPB11, HRPB11B, MGC54043, MGC105050; RPB11b1
• Summary: This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]