Human Separase (ESPL1) activation kit by CRISPRa

SKU GA106511

ESPL1 CRISPRa kit - CRISPR gene activation of human extra spindle pole bodies like 1, separase

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: ESPL1
• Locus ID: 9700
• Components:

  GA106511G1, Separase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA106511G2, Separase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA106511G3, Separase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012291
• UniProt ID: Q14674
• Synonyms: ESP1; SEPA
• Summary: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]