Separase (ESPL1) (NM_012291) Human Tagged ORF Clone

SKU RC217230

ESPL1 (Myc-DDK-tagged)-Human extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Separase
• Synonyms: ESP1; SEPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217230 representing NM_012291; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGAGCTTCAAAAGAGTCAACTTTGGGACTCTGCTAAGCAGCCAGAAGGAGGCTGAAGAGTTGCTGC; CCGCCTTGAAGGAGTTCCTGTCCAACCCTCCAGCTGGTTTTCCCAGCAGCCGATCTGATGCTGAGAGGAG; ACAAGCTTGTGATGCCATCCTGAGGGCTTGCAACCAGCAGCTGACTGCTAAGCTAGCTTGCCCTAGGCAT; CTGGGGAGCCTGCTGGAGCTGGCAGAGCTGGCCTGTGATGGCTACTTAGTGTCTACCCCACAGCGTCCTC; CCCTCTACCTGGAACGAATTCTCTTTGTCTTACTGCGGAATGCTGCTGCACAAGGAAGCCCAGAGGCCAC; ACTCCGCCTTGCTCAGCCCCTCCATGCCTGCTTGGTGCAGTGCTCTCGCGAGGCTGCTCCCCAGGACTAT; GAGGCCGTGGCTCGGGGCAGCTTTTCTCTGCTTTGGAAGGGGGCAGAAGCCCTGTTGGAACGGCGAGCTG; CATTTGCAGCTCGGCTGAAGGCCTTGAGCTTCCTAGTACTCTTGGAGGATGAAAGTACCCCTTGTGAGGT; TCCTCACTTTGCTTCTCCAACAGCCTGTCGAGCGGTAGCTGCCCATCAGCTATTTGATGCCAGTGGCCAT; GGTCTAAATGAAGCAGATGCTGATTTCCTAGATGACCTGCTCTCCAGGCACGTGATCAGAGCCTTGGTGG; GTGAGAGAGGGAGCTCTTCTGGGCTTCTTTCTCCCCAGAGGGCCCTCTGCCTCTTGGAGCTCACCTTGGA; ACACTGCCGTCGCTTTTGCTGGAGCCGCCACCATGACAAAGCCATCAGCGCAGTGGAGAAGGCTCACAGT; TACCTAAGGAACACCAATCTAGCCCCTAGCCTTCAGCTATGTCAGCTGGGGGTTAAGCTGCTGCAGGTTG; GGGAGGAAGGACCTCAGGCAGTGGCCAAGCTTCTGATCAAGGCATCAGCTGTCCTGAGCAAGAGTATGGA; GGCACCATCACCCCCACTTCGGGCATTGTATGAGAGCTGCCAGTTCTTCCTTTCAGGCCTGGAACGAGGC; ACCAAGAGGCGCTATAGACTTGATGCCATTCTGAGCCTCTTTGCTTTTCTTGGAGGGTACTGCTCTCTTC; TGCAGCAGCTGCGGGATGATGGTGTGTATGGGGGCTCCTCCAAGCAACAGCAGTCTTTTCTTCAGATGTA; CTTTCAGGGACTTCACCTCTACACTGTGGTGGTTTATGACTTTGCCCAAGGCTGTCAGATAGTTGATTTG; GCTGACCTGACCCAACTAGTGGACAGTTGTAAATCTACCGTTGTCTGGATGCTGGAGGCCTTAGAGGGCC; TGTCGGGCCAAGAGCTGACGGACCACATGGGGATGACCGCTTCTTACACCAGTAATTTGGCCTACAGCTT; CTATAGTCACAAGCTCTATGCCGAGGCCTGTGCCATCTCTGAGCCGCTCTGTCAGCACCTGGGTTTGGTG; AAGCCAGGCACTTATCCCGAGGTGCCTCCTGAGAAGTTGCACAGGTGCTTCCGGCTACAAGTAGAGAGTT; TGAAGAAACTGGGTAAACAGGCCCAGGGCTGCAAGATGGTGATTTTGTGGCTGGCAGCCCTGCAACCCTG; TAGCCCTGAACACATGGCTGAGCCAGTCACTTTCTGGGTTCGGGTCAAGATGGATGCGGCCAGGGCTGGA; GACAAGGAGCTACAGCTAAAGACTCTGCGAGACAGCCTCAGTGGCTGGGACCCGGAGACCCTGGCCCTCC; TGCTGAGGGAGGAGCTGCAGGCCTACAAGGCGGTGCGGGCCGACACTGGACAGGAACGCTTCAACATCAT; CTGTGACCTCCTGGAGCTGAGCCCCGAGGAGACACCAGCCGGGGCCTGGGCACGAGCCACCCACCTGGTA; GAACTGGCTCAGGTGCTCTGCTACCACGACTTTACGCAGCAGACCAACTGCTCTGCTCTGGATGCTATCC; GGGAAGCCCTGCAGCTTCTGGACTCTGTGAGGCCTGAGGCCCAGGCCAGAGATCAGCTTCTGGACGATAA; AGCACAGGCCTTGCTGTGGCTTTACATCTGTACTCTGGAAGCCAAAATGCAGGAAGGTATCGAGCGGGAT; CGGAGAGCCCAGGCCCCTGGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAAGATAAACTCC; AGGAAGATCGTTTCCTATACAGTAACATTGCCTTCAACCTGGCTGCAGATGCTGCTCAGTCCAAATGCCT; GGACCAAGCCCTGGCCCTGTGGAAGGAGCTGCTTACAAAGGGGCAGGCCCCAGCTGTACGGTGTCTCCAG; CAGACAGCAGCCTCACTGCAGATCCTAGCAGCCCTCTACCAGCTGGTGGCAAAGCCCATGCAGGCTCTGG; AGGTCCTCCTGCTGCTACGGATTGTCTCTGAGAGACTGAAGGACCACTCGAAGGCAGCTGGCTCCTCCTG; CCACATCACCCAGCTCCTCCTGACCCTCGGCTGTCCCAGCTATGCCCAGTTACACCTGGAAGAGGCAGCA; TCGAGCCTGAAGCATCTCGATCAGACTACTGACACATACCTGCTCCTTTCCCTGACCTGTGATCTGCTTC; GAAGTCAACTCTACTGGACTCACCAGAAGGTGACCAAGGGTGTCTCTCTGCTGCTGTCTGTGCTTCGGGA; TCCTGCCCTCCAGAAGTCCTCCAAGGCTTGGTACTTGCTGCGTGTCCAGGTCCTGCAGCTGGTGGCAGCT; TACCTTAGCCTCCCGTCAAACAACCTCTCACACTCCCTGTGGGAGCAGCTCTGTGCCCAAGGCTGGCAGA; CACCTGAGATAGCTCTCATAGACTCCCATAAGCTCCTCCGAAGCATCATCCTCCTGCTGATGGGCAGTGA; CATTCTCTCAACTCAGAAAGCAGCTGTGGAGACATCGTTTTTGGACTATGGTGAAAATCTGGTACAAAAA; TGGCAGGTTCTTTCAGAGGTGCTGAGCTGCTCAGAGAAGCTGGTCTGCCACCTGGGCCGCCTGGGTAGTG; TGAGTGAAGCCAAGGCCTTTTGCTTGGAGGCCCTAAAACTTACAACAAAGCTGCAGATACCACGCCAGTG; TGCCCTGTTCCTGGTGCTGAAGGGCGAGCTGGAGCTGGCCCGCAATGACATTGATCTCTGTCAGTCGGAC; CTGCAGCAGGTTCTGTTCTTGCTTGAGTCTTGCACAGAGTTTGGTGGGGTGACTCAGCACCTGGACTCTG; TGAAGAAGGTCCACCTGCAGAAGGGGAAGCAGCAGGCCCAGGTCCCCTGTCCTCCACAGCTCCCAGAGGA; GGAGCTCTTCCTAAGAGGCCCTGCTCTAGAGCTGGTGGCCACTGTGGCCAAGGAGCCTGGCCCCATAGCA; CCTTCTACAAACTCCTCCCCAGTCTTGAAAACCAAGCCCCAGCCCATACCCAACTTCCTGTCCCATTCAC; CCACCTGTGACTGCTCGCTCTGCGCCAGCCCTGTCCTCACAGCAGTCTGTCTGCGCTGGGTATTGGTCAC; GGCAGGGGTGAGGCTGGCCATGGGCCACCAAGCCCAGGGTCTGGATCTGCTGCAGGTCGTGCTGAAGGGC; TGTCCTGAAGCCGCTGAGCGCCTCACCCAAGCTCTCCAAGCTTCCCTGAATCATAAAACACCCCCCTCCT; TGGTTCCAAGCCTCTTGGATGAGATCTTGGCTCAAGCATACACACTGTTGGCACTGGAGGGCCTGAACCA; GCCATCAAACGAGAGCCTGCAGAAGGTTCTACAGTCAGGGCTGAAGTTTGTAGCAGCACGGATACCCCAC; CTAGAGCCCTGGCGAGCCAGCCTGCTCTTGATTTGGGCCCTCACAAAACTAGGTGGCCTCAGCTGCTGTA; CTACCCAACTTTTTGCAAGCTCCTGGGGCTGGCAGCCACCATTAATAAAAAGTGTCCCTGGCTCAGAGCC; CTCTAAGACTCAGGGCCAAAAACGTTCTGGACGAGGGCGCCAAAAGTTAGCCTCTGCTCCCCTGCGCCTC; AATAATACCTCTCAGAAAGGTCTGGAAGGTAGAGGACTGCCCTGCACACCTAAACCCCCAGACCGGATCA; GGCAAGCTGGCCCTCATGTCCCCTTCACGGTGTTTGAGGAAGTCTGCCCTACAGAGAGCAAGCCTGAAGT; ACCCCAGGCCCCCAGGGTACAACAGAGAGTCCAGACGCGCCTCAAGGTGAACTTCAGTGATGACAGTGAC; TTGGAAGACCCTGTCTCAGCTGAGGCCTGGCTGGCAGAGGAGCCTAAGAGACGGGGCACTGCTTCCCGGG; GCCGGGGGCGAGCAAGGAAGGGCCTGAGCCTAAAGACGGATGCCGTGGTTGCCCCAGGTAGTGCCCCTGG; GAACCCTGGCCTGAATGGCAGGAGCCGGAGGGCCAAGAAGGTGGCATCAAGACATTGTGAGGAGCGGCGT; CCCCAGAGGGCCAGTGACCAGGCCAGGCCTGGCCCTGAGATCATGAGGACCATCCCTGAGGAAGAACTGA; CTGACAACTGGAGAAAAATGAGCTTTGAGATCCTCAGGGGCTCTGACGGGGAAGACTCAGCCTCAGGTGG; GAAGACTCCAGCTCCGGGCCCTGAGGCAGCTTCTGGAGAATGGGAGCTGCTGAGGCTGGATTCCAGCAAG; AAGAAGCTGCCCAGCCCATGCCCAGACAAGGAGAGTGACAAGGACCTTGGTCCTCGGCTCCGGCTCCCCT; CAGCCCCCGTAGCCACTGGTCTTTCTACCCTGGACTCCATCTGTGACTCCCTGAGTGTTGCTTTCCGGGG; CATTAGTCACTGTCCTCCTAGTGGGCTCTATGCCCACCTCTGCCGCTTCCTGGCCTTGTGCCTGGGCCAC; CGGGATCCTTATGCCACTGCTTTCCTTGTCACCGAGTCTGTCTCCATCACCTGTCGCCACCAGCTGCTCA; CCCACCTCCACAGACAGCTCAGCAAGGCCCAGAAGCACCGAGGATCACTTGAAATAGCAGACCAGCTGCA; GGGGCTGAGCCTTCAGGAGATGCCTGGAGATGTCCCCCTGGCCCGCATCCAGCGCCTCTTTTCCTTCAGG; GCTTTGGAATCTGGCCACTTCCCCCAGCCTGAAAAGGAGAGTTTCCAGGAGCGCCTGGCTCTGATCCCCA; GTGGGGTGACTGTGTGTGTGTTGGCCCTGGCCACCCTCCAGCCCGGAACCGTGGGCAACACCCTCCTGCT; GACCCGGCTGGAAAAGGACAGTCCCCCAGTCAGTGTGCAGATTCCCACTGGCCAGAACAAGCTTCATCTG; CGTTCAGTCCTGAATGAGTTTGATGCCATCCAGAAGGCACAGAAAGAGAACAGCAGCTGTACTGACAAGC; GAGAATGGTGGACAGGGCGGCTGGCACTGGACCACAGGATGGAGGTTCTCATCGCTTCCCTAGAGAAGTC; TGTGCTGGGCTGCTGGAAGGGGCTGCTGCTGCCGTCCAGTGAGGAGCCCGGCCCTGCCCAGGAGGCCTCC; CGCCTACAGGAGCTGCTACAGGACTGTGGCTGGAAATATCCTGACCGCACTCTGCTGAAAATCATGCTCA; GTGGTGCCGGTGCCCTCACCCCTCAGGACATTCAGGCCCTGGCCTACGGGCTGTGCCCAACCCAGCCAGA; GCGAGCCCAGGAGCTCCTGAATGAGGCAGTAGGACGTCTACAGGGCCTGACAGTACCAAGCAATAGCCAC; CTTGTCTTGGTCCTAGACAAGGACTTGCAGAAGCTGCCGTGGGAAAGCATGCCCAGCCTCCAAGCACTGC; CTGTCACCCGGCTGCCCTCCTTCCGCTTCCTACTCAGCTACTCCATCATCAAAGAGTATGGGGCCTCGCC; AGTGCTGAGTCAAGGGGTGGATCCACGAAGTACCTTCTATGTCCTGAACCCTCACAATAACCTGTCAAGC; ACAGAGGAGCAATTTCGAGCCAATTTCAGCAGTGAAGCTGGCTGGAGAGGAGTGGTTGGGGAGGTGCCAA; GACCTGAACAGGTGCAGGAAGCCCTGACAAAGCATGATTTGTATATCTATGCAGGGCATGGGGCTGGTGC; CCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAGCTGTCGGGCAGTGGCCCTGCTGTTTGGCTGTAGC; AGTGCGGCCCTGGCTGTGCGTGGAAACCTGGAGGGGGCTGGCATCGTGCTCAAGTACATCATGGCTGGTT; GCCCCTTGTTTCTGGGTAATCTCTGGGATGTGACTGACCGCGACATTGACCGCTACACGGAAGCTCTGCT; GCAAGGCTGGCTTGGAGCAGGCCCAGGGGCCCCCCTTCTCTACTATGTAAACCAGGCCCGCCAAGCTCCC; CGACTCAAGTATCTTATTGGGGCTGCACCTATAGCCTATGGCTTGCCTGTCTCTCTGCGG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217230 representing NM_012291; Red=Cloning site Green=Tags(s); ; MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRH; LGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDY; EAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGH; GLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHS; YLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERG; TKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDL; ADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLV; KPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAG; DKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLV; ELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERD; RRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQ; QTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAA; SSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAA; YLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQK; WQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSD; LQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIA; PSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKG; CPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPH; LEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRL; NNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSD; LEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERR; PQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSK; KKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGH; RDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFR; ALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHL; RSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEAS; RLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSH; LVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSS; TEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCS; SAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAP; RLKYLIGAAPIAYGLPVSLR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012291
• ORF Size: 6360 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012291.5
• RefSeq Size: 6641 bp
• RefSeq ORF: 6363 bp
• Locus ID: 9700
• UniProt ID: Q14674
• Cytogenetics: 12q13.13
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Cell cycle, Oocyte meiosis
• MW: 233.2 kDa
• Summary: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]