OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Human SIAH1 activation kit by CRISPRa

Be the first to review this product
SKU
GA104408
SIAH1 CRISPRa kit - CRISPR gene activation of human siah E3 ubiquitin protein ligase 1
  $1,657.00
4 Weeks*

Frequently Bought Together (1)

RC206576 SIAH1 (Myc-DDK-tagged)-Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1
10 ug
$450.00

Other products for "SIAH1"

Specifications
Specifications
Product Data
Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Target Symbol SIAH1
Locus ID 6477
Components

GA104408G1, SIAH1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

GA104408G2, SIAH1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

GA104408G3, SIAH1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

1 CRISPRa-Enhancer vector, SKU GE100056

1 CRISPRa scramble vector, SKU GE100077
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
Shipping Ambient
Reference Data
RefSeq NM_001006610, NM_001006611, NM_003031
UniProt ID Q8IUQ4
Synonyms SIAH1A
Summary This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. provided by RefSeq, Jul 2008
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other Versions
SKU Description Size Price
KN206576 SIAH1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN206576BN SIAH1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN206576LP SIAH1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN206576RB SIAH1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN406576 SIAH1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin
Izumi, Nakato, Zhang et al
Nat. Genet. (2015) 47 (4), 338-44 DOI: 10.1038/ng.3229
Cited by 149
AI Summary: OriGene products contributed to this study by providing Myc-DDK-tagged AFF4 (RC217692) and Myc-DDK-tagged SIAH1a (RC206576) plasmids, as well as the RapidShuttling kit (PS200008) used for generating tagged constructs, which facilitated functional analyses of the implicated proteins in the described developmental syndrome.
Snippet: … Antibodies to ELL2 used for immunoblotting (A302-505A) and AFF4 used for ChIP-seq analysis (A302-538A) were purchased from Bethyl Lab. Antibodies to NIPBL (sc-374625) and cyclin T1 (sc-10750) were from Santa Cruz Biotechnology. …Plasmids encoding MycDDK-tagged AFF4 (RC217692) and Myc-DDK-tagged SIAH1a (RC206576) were obtained from OriGene Technologies. To generate HA-His-tagged SIAH1a, the SIAH1A cDNA was subcloned into pCMV6-AC-HA-His, using the OriGene RapidShuttling kit (SfgI-MluI, PS200008) according to the manufacturer’s directions. Mutagenesis of the AFF4 expression plasmid to introduce each of the nonsynonymous variants was performed using the QuikChange Site-Directed Mutagenesis kit by Agilent Technologies following the manufacturer’s protocol. …
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products