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NS5a (NC_009824) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102208
Myc-DDK-tagged ORF clone of viral ORF for NS5a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491556
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol NS5a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102208 represents NCBI reference of YP_001491556 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGATGATTGGCTCAGGACCATCTGGGACTGGGTCTGCAGTGTGCTTGCCGACTTTAAGGCCTGGC
TCAGTGCTAAGATCATGCCGGCTCTTCCTGGCCTGCCCTTCATTTCCTGCCAGAAAGGGTACAAGGGAGT
CTGGAGAGGCGACGGGGTTATGAGCACGCGATGCCCATGCGGCGCGGCAATCACAGGCCACGTTAAAAAT
GGCTCAATGAGACTGGCTGGACCCCGCACTTGCGCAAATATGTGGCACGGCACATTCCCTATAAATGAGT
ATACTACCGGCCCCTCCACCCCCTGCCCAAGCCCAAACTACACCAGAGCTCTGTGGAGGGTCGCCGCAAA
TTCATATGTGGAGGTGAGGAGAGTGGGGGACTTTCACTACATCACAGGCGCAACTGAAGACGAACTGAAA
TGCCCGTGCCAGGTTCCCGCCGCCGAGTTTTTCACAGAGGTCGACGGAGTAAGACTGCACCGGTACGCCC
CTCCATGCAAGCCACTGTTGCGGGACGACATTACTTTTATGGTAGGACTCCATTCCTATACAATCGGATC
ACAACTTCCTTGCGAGCCAGAACCCGATGTGTCCGTGCTGACAAGTATGCTCCGAGATCCATCCCATATT
ACCGCTGAGACAGCCGCTAGACGGCTGGCCCGGGGCAGTCCTCCCAGCGAGGCAAGTAGTTCTGCAAGTC
AACTGTCTGCACCCTCCCTCAAGGCCACTTGTCAGACCCACCGGCCTCACCCTGATGCCGAGCTGGTTGA
CGCCAACCTTCTTTGGCGGCAGGAGATGGGTAGTAATATTACCCGAGTGGAATCTGAGACTAAAGTAGTC
GTCTTGGACTCCTTCGAACCTCTGAGAGCAGAAACAGACGATGTCGAGCCAAGTGTTGCCGCCGAATGTT
TTAAGAAGCCACCCAAATATCCCCCTGCGCTCCCCATTTGGGCTCGGCCGGACTACAACCCACCACTCCT
GGATAGGTGGAAGGCTCCCGACTATGTGCCCCCTACGGTTCACGGTTGTGCTCTGCCTCCTCGAGGAGCT
CCTCCTGTCCCTCCTCCCAGGCGCAAAAGGACCATTCAACTGGACGGGAGTAACGTGAGCGCCGCACTTG
CCGCCTTGGCAGAGAAATCTTTTCCTTCCAGTAAGCCACAGGAAGAGAACAGTAGCTCCAGCGGGGTAGA
TACTCAAAGCTCCACCACATCTAAAGTTCCTCCTTCTCCTGGCGGTGAGAGTGACTCCGAGTCATGTTCA
TCCATGCCCCCGTTGGAAGGGGAGCCCGGTGACCCTGATCTGAGTTGCGACAGTTGGTCCACAGTATCTG
ACAGTGAAGAGCAGAGTGTAGTGTGCTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102208 representing YP_001491556
Red=Cloning sites Green=Tags

MSDDWLRTIWDWVCSVLADFKAWLSAKIMPALPGLPFISCQKGYKGVWRGDGVMSTRCPCGAAITGHVKN
GSMRLAGPRTCANMWHGTFPINEYTTGPSTPCPSPNYTRALWRVAANSYVEVRRVGDFHYITGATEDELK
CPCQVPAAEFFTEVDGVRLHRYAPPCKPLLRDDITFMVGLHSYTIGSQLPCEPEPDVSVLTSMLRDPSHI
TAETAARRLARGSPPSEASSSASQLSAPSLKATCQTHRPHPDAELVDANLLWRQEMGSNITRVESETKVV
VLDSFEPLRAETDDVEPSVAAECFKKPPKYPPALPIWARPDYNPPLLDRWKAPDYVPPTVHGCALPPRGA
PPVPPPRRKRTIQLDGSNVSAALAALAEKSFPSSKPQEENSSSSGVDTQSSTTSKVPPSPGGESDSESCS
SMPPLEGEPGDPDLSCDSWSTVSDSEEQSVVCC

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_009824
ORF Size 1359 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_009824.1, YP_001491556
RefSeq ORF 1359 bp
MW 49.2 kDa
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"NC_009824" in other vectors (8)
SKU Description Size Price
VC102201 Myc-DDK-tagged ORF clone of viral ORF for C [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491549 10 ug
$330.00
VC102202 Myc-DDK-tagged ORF clone of viral ORF for E1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491550 10 ug
$330.00
VC102203 Myc-DDK-tagged ORF clone of viral ORF for E2/NS1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491551 10 ug
$503.00
VC102204 Myc-DDK-tagged ORF clone of viral ORF for NS2 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491552 10 ug
$330.00
VC102205 Myc-DDK-tagged ORF clone of viral ORF for NS3 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491553 10 ug
$910.00
VC102206 Myc-DDK-tagged ORF clone of viral ORF for NS4a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491554 10 ug
$165.00
VC102207 Myc-DDK-tagged ORF clone of viral ORF for NS4b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491555 10 ug
$330.00
VC102209 Myc-DDK-tagged ORF clone of viral ORF for NS5b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491557 10 ug
$606.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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