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NS3 (NC_009824) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102205
Myc-DDK-tagged ORF clone of viral ORF for NS3 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491553
  $910.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol NS3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102205 represents NCBI reference of YP_001491553 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAGAGAAGTCCTGCTTGGTCCAGCCGACGATTACAGGGAGATGGGGTGGCGCCTTCTGGCACCAA
TTACGGCTTATGCTCAGCAAACACGCGGTCTCCTCGGTACAATTGTGACCAGTCTTACTGGTAGGGATAA
GAATGTCGTAACAGGCGAGGTGCAGGTCTTGAGCACCGCCACCCAAACTTTCCTGGGAACAACAGTGGGT
GGAGTCATTTGGACGGTGTACCACGGTGCAGGCTCAAGAACCCTTGCCGGCGCAAAACACCCGGCCCTTC
AGATGTACACAAACGTGGACCAGGACCTTGTAGGCTGGCCGGCCCCACCGGGCGCTAAGTCACTCGAACC
TTGCGCCTGTGGCTCATCTGATCTTTACTTGGTGACCAGAGACGCCGACGTTATTCCAGCAAGAAGGAGG
GGAGATTCCACTGCATCCCTGCTGAGCCCCCGCCCATTGGCGTGCCTGAAAGGTTCCAGCGGCGGACCTG
TTATGTGCCCCTCCGGCCACGTCGCGGGCATTTTCCGAGCAGCCGTATGTACAAGAGGCGTGGCAAAATC
CCTCCAATTTATTCCTGTGGAGACGTTGTCTACACAGGCAAGGAGCCCCTCCTTTAGCGACAACAGCACG
CCTCCAGCCGTGCCTCAGTCTTATCAGGTTGGCTATCTCCACGCCCCTACGGGGTCTGGCAAAAGTACGA
AGGTCCCAGCAGCATACGTGGCACAGGGCTACAACGTTCTGGTTCTGAATCCTTCAGTCGCAGCTACCCT
TGGCTTTGGATCTTTCATGTCCAGGGCCTATGGCATCGATCCTAACATTCGGACAGGTAACAGAACTGTC
ACAACAGGAGCGAAGTTGACCTACTCTACCTACGGCAAGTTTCTTGCCGATGGGGGATGTAGTGGGGGTG
CTTACGACGTCATTATTTGTGATGAATGTCACGCTCAGGATGCAACCTCTATTCTCGGAATCGGCACTGT
CCTGGACCAGGCAGAGACCGCTGGAGTGCGGCTGACAGTGCTGGCCACAGCCACCCCTCCAGGTAGTATC
ACAGTCCCACATTCCAACATTGAAGAGGTTGCGCTGGGCTCTGAGGGCGAGATACCATTTTATGGAAAGG
CCATCCCAATAGCTCTCCTGAAAGGAGGTCGGCACCTTATTTTCTGCCACTCCAAAAAGAAATGCGATGA
GATCGCAAGTAAGCTGCGGGGAATGGGACTCAACGCCGTCGCATACTACCGGGGCCTGGACGTGAGTGTA
ATCCCCACCACTGGAGATGTGGTGGTATGCGCCACTGATGCCCTCATGACTGGCTTCACCGGCGACTTCG
ATTCAGTGATCGATTGCAACGTAGCCGTCGAGCAGTACGTCGACTTCTCTCTGGATCCAACCTTCTCTAT
CGAAACACGGACAGCCCCACAAGATGCCGTCTCCCGATCTCAAAGACGCGGCCGGACAGGGAGAGGCCGA
CTCGGCACATACAGATATGTGGCCAGCGGAGAGCGACCCAGCGGCATGTTCGATTCAGTTGTGCTGTGCG
AGTGTTATGACGCCGGATGTTCTTGGTATGATCTGCAGCCGGCTGAAACTACTGTTAGACTCCGGGCTTA
TCTGTCCACCCCAGGGTTGCCCGTTTGTCAGGACCACCTCGATTTCTGGGAGTCTGTATTCACCGGCCTG
ACTCACATCGACGCTCACTTTCTGTCCCAAACGAAGCAACAGGGTTTGAACTTCAGTTATCTGACTGCAT
ACCAGGCTACAGTTTGTGCTCGGGCACAGGCGCCCCCACCTTCTTGGGATGAAATGTGGAAGTGCCTTGT
ACGGCTTAAACCCACTCTCCATGGCCCGACCCCTTTGCTGTATCGCTTGGGTCCAGTTCAGAACGAAACT
TGTCTGACTCATCCCATCACCAAGTATCTTATGGCCTGCATGAGCGCCGATCTGGAGGTCACTACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102205 representing YP_001491553
Red=Cloning sites Green=Tags

MGREVLLGPADDYREMGWRLLAPITAYAQQTRGLLGTIVTSLTGRDKNVVTGEVQVLSTATQTFLGTTVG
GVIWTVYHGAGSRTLAGAKHPALQMYTNVDQDLVGWPAPPGAKSLEPCACGSSDLYLVTRDADVIPARRR
GDSTASLLSPRPLACLKGSSGGPVMCPSGHVAGIFRAAVCTRGVAKSLQFIPVETLSTQARSPSFSDNST
PPAVPQSYQVGYLHAPTGSGKSTKVPAAYVAQGYNVLVLNPSVAATLGFGSFMSRAYGIDPNIRTGNRTV
TTGAKLTYSTYGKFLADGGCSGGAYDVIICDECHAQDATSILGIGTVLDQAETAGVRLTVLATATPPGSI
TVPHSNIEEVALGSEGEIPFYGKAIPIALLKGGRHLIFCHSKKKCDEIASKLRGMGLNAVAYYRGLDVSV
IPTTGDVVVCATDALMTGFTGDFDSVIDCNVAVEQYVDFSLDPTFSIETRTAPQDAVSRSQRRGRTGRGR
LGTYRYVASGERPSGMFDSVVLCECYDAGCSWYDLQPAETTVRLRAYLSTPGLPVCQDHLDFWESVFTGL
THIDAHFLSQTKQQGLNFSYLTAYQATVCARAQAPPPSWDEMWKCLVRLKPTLHGPTPLLYRLGPVQNET
CLTHPITKYLMACMSADLEVTT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_009824
ORF Size 1956 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_009824.1, YP_001491553
RefSeq ORF 1956 bp
MW 69.5 kDa
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Documents
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"NC_009824" in other vectors (8)
SKU Description Size Price
VC102201 Myc-DDK-tagged ORF clone of viral ORF for C [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491549 10 ug
$330.00
VC102202 Myc-DDK-tagged ORF clone of viral ORF for E1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491550 10 ug
$330.00
VC102203 Myc-DDK-tagged ORF clone of viral ORF for E2/NS1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491551 10 ug
$503.00
VC102204 Myc-DDK-tagged ORF clone of viral ORF for NS2 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491552 10 ug
$330.00
VC102206 Myc-DDK-tagged ORF clone of viral ORF for NS4a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491554 10 ug
$165.00
VC102207 Myc-DDK-tagged ORF clone of viral ORF for NS4b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491555 10 ug
$330.00
VC102208 Myc-DDK-tagged ORF clone of viral ORF for NS5a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491556 10 ug
$503.00
VC102209 Myc-DDK-tagged ORF clone of viral ORF for NS5b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491557 10 ug
$606.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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