Lamin A (LMNA) (NM_170707) Human Tagged ORF Clone
$1,127.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Lamin A |
| Synonyms | CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_170707 |
| ORF Size | 1992 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_170707.4 |
| RefSeq Size | 3181 bp |
| RefSeq ORF | 1995 bp |
| Locus ID | 4000 |
| UniProt ID | P02545 |
| Cytogenetics | 1q22 |
| Protein Families | Druggable Genome |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM) |
| Summary | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. provided by RefSeq, Apr 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_170707" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204970 | LMNA (Myc-DDK-tagged)-Human lamin A/C (LMNA), transcript variant 1 | 10 ug |
$927.00
|
|
| RC204970L1 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,227.00
|
|
| RC204970L2 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, mGFP tagged | 10 ug |
$1,227.00
|
|
| RC204970L3 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,227.00
|
|
| RC204970L4 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, mGFP tagged | 10 ug |
$1,227.00
|
|
| SC101048 | LMNA (untagged)-Human lamin A/C (LMNA), transcript variant 1 | 10 ug |
$619.00
|
|
Citations
Metformin alters peripheral blood mononuclear cells (PBMC) senescence biomarkers gene expression in type 2 diabetic patients
Al Dubayee, Alshahrani, Almalk et al
J Diabetes Complications (2021) 35 (1), 107758 DOI: 10.1016/j.jdiacomp.2020.107758
Al Dubayee, Alshahrani, Almalk et al
J Diabetes Complications (2021) 35 (1), 107758 DOI: 10.1016/j.jdiacomp.2020.107758
Cited by 16
AI Summary: OriGene products, specifically LMNA (RC209875) and LMNC (SC324349) expression plasmids, were used for ectopic expression in human monocytic cell lines (THP-1 and U937) to investigate the impact of these genes on inflammatory mediator expression in the study.
Whole-exome sequencing to identify a novel LMNA gene mutation associated with inherited cardiac conduction disease
Lai, Yeh, Hsieh et al
PLoS One (2013) 8 (12), e83322 DOI: 10.1371/journal.pone.0083322
Lai, Yeh, Hsieh et al
PLoS One (2013) 8 (12), e83322 DOI: 10.1371/journal.pone.0083322
Cited by 14
AI Summary: OriGene products contributed to this study by providing the LMNA cDNA clone, which was used to express both wild-type and mutant LMNA proteins in cellular models to assess the pathogenicity of the novel LMNA mutation associated with inherited cardiac conduction disease.
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
