Lamin A (LMNA) (NM_170707) Human Untagged Clone
$619.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | Lamin A |
| Synonyms | CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_170707 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_170707.1, NP_733821.1 |
| RefSeq Size | 3181 bp |
| RefSeq ORF | 1995 bp |
| Locus ID | 4000 |
| UniProt ID | P02545 |
| Cytogenetics | 1q22 |
| Protein Families | Druggable Genome |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM) |
| Summary | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. provided by RefSeq, Apr 2012 Transcript Variant: This variant (1) encodes isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
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Resources
"NM_170707" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204970 | LMNA (Myc-DDK-tagged)-Human lamin A/C (LMNA), transcript variant 1 | 10 ug |
$927.00
|
|
| RC204970L1 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,227.00
|
|
| RC204970L2 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, mGFP tagged | 10 ug |
$1,227.00
|
|
| RC204970L3 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,227.00
|
|
| RC204970L4 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, mGFP tagged | 10 ug |
$1,227.00
|
|
| RG204970 | LMNA (tGFP-tagged) - Human lamin A/C (LMNA), transcript variant 1 | 10 ug |
$1,127.00
|
|
Citations
Disrupting the LINC complex in smooth muscle cells reduces aortic disease in a mouse model of Hutchinson-Gilford progeria syndrome
Kim, Luu, Heizer et al
Sci Transl Med (2018) 10 (460) DOI: 10.1126/scitranslmed.aat7163
Kim, Luu, Heizer et al
Sci Transl Med (2018) 10 (460) DOI: 10.1126/scitranslmed.aat7163
Cited by 82
AI Summary: In this study, OriGene’s pCMV6-Entry-Lmna and pCMV6-Entry-Sun1 plasmids were used to express relevant genes in smooth muscle cells, enabling the authors to investigate the effects of disrupting the LINC complex on aortic disease in a mouse model of Hutchinson-Gilford progeria syndrome.
Fibroblasts lacking nuclear lamins do not have nuclear blebs or protrusions but nevertheless have frequent nuclear membrane ruptures
Chen, Kim, Weston et al
PNAS (2018)
Chen, Kim, Weston et al
PNAS (2018)
AI Summary: OriGene products, specifically the human prelamin A cDNA (#SC101048), were used to generate expression vectors for nuclear lamin studies in this paper, enabling the investigation of how the absence of lamins affects nuclear envelope integrity in fibroblasts.
Showing 1-2 of 2 papers.
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