Lamin A (LMNA) (NM_170707) Human Tagged ORF Clone
$927.00
5 Days*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Lamin A |
| Synonyms | CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_170707 |
| ORF Size | 1992 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_170707.4 |
| RefSeq Size | 3239 bp |
| RefSeq ORF | 1995 bp |
| Locus ID | 4000 |
| UniProt ID | P02545, A0A384MQX1 |
| Cytogenetics | 1q22 |
| Protein Families | Druggable Genome |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM) |
| MW | 74.1 kDa |
| Summary | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. provided by RefSeq, Apr 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_170707" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204970L1 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,227.00
|
|
| RC204970L2 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, mGFP tagged | 10 ug |
$1,227.00
|
|
| RC204970L3 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,227.00
|
|
| RC204970L4 | Lenti ORF clone of Human lamin A/C (LMNA), transcript variant 1, mGFP tagged | 10 ug |
$1,227.00
|
|
| RG204970 | LMNA (tGFP-tagged) - Human lamin A/C (LMNA), transcript variant 1 | 10 ug |
$1,127.00
|
|
| SC101048 | LMNA (untagged)-Human lamin A/C (LMNA), transcript variant 1 | 10 ug |
$619.00
|
|
Citations
Loss of Mature Lamin A/C Triggers a Shift in Intracellular Metabolic Homeostasis via AMPKα Activation
Zhou, Yang, Cheng et al
Cells (2022) 11 (24) DOI: 10.3390/cells11243988
Zhou, Yang, Cheng et al
Cells (2022) 11 (24) DOI: 10.3390/cells11243988
Cited by 4
AI Summary: OriGene products, specifically the untagged human LMNA (lamin C) clone (#SC321549), the tagged human LMNA (lamin A) clone (#RC204970), and the pCMV6-AC vector (PS100020), were used to generate wild-type and mutant LMNA constructs for functional studies investigating the role of lamin A/C in cellular metabolism.
Snippet: the LMNA cDNA and Pre–LMNA and Mutant Plasmids The untagged human LMNA (lamin C) (NM_005572) clone (#SC321549) and the tagged human LMNA (lamin A) (NM_170707) Clone (#RC204970) were purchased from OriGene Technologies (Jiangsu, China). PCR products containing the LMNA/C cDNA or different mutations were purified, sequenced (Rui Bo Xing Ke Company, Beijing, China) and then cloned into the pCMV6-A;The untagged human LMNA (lamin C) (NM_005572) clone (#SC321549) and the tagged human LMNA (lamin A) (NM_170707) Clone (#RC204970) were purchased from OriGene Technologies (Jiangsu, China). PCR products containing the LMNA/C cDNA or different mutations were purified, sequenced (Rui Bo Xing Ke Company, Beijing, China) and then cloned into the pCMV6-AC vector (PS100020, OriGene)
Genotype-phenotype analysis of LMNA-related diseases predicts phenotype-selective alterations in lamin phosphorylation
Lin, Brady, Kwan et al
FASEB J. (2020)
Lin, Brady, Kwan et al
FASEB J. (2020)
AI Summary: OriGene products, specifically the human LMNA open-reading frame and the pCMV6 vector with an amino-terminal myc/DDK tag, were used to generate and express wild-type and mutant LMNA constructs for experimental validation in this study.
Snippet: Human LMNA open‐reading frame was purchased from Origene (Rockville, MD) and subcloned into the pCMV6 vector with amino‐terminal myc/DDK tag (Origene) as described.47 LMNA mutants were generated using the QuikChange II site‐directed mutagenesis;- Human LMNA open‐reading frame was purchased from Origene (Rockville, MD) and subcloned into the pCMV6 vector with amino‐terminal myc/DDK tag (Origene)...
Lamin A/C might be involved in the EMT signalling pathway
Zuo, Zhao, Yang et al
Gene (2018)
Zuo, Zhao, Yang et al
Gene (2018)
Showing 1-3 of 3 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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