PRMT1 (NM_001536) Human Tagged ORF Clone
SKU
RC224239
PRMT1 (Myc-DDK-tagged)-Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PRMT1 |
| Synonyms | ANM1; HCP1; HRMT1L2; IR1B4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001536 |
| ORF Size | 1113 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001536.6 |
| RefSeq Size | 1386 bp |
| RefSeq ORF | 1116 bp |
| Locus ID | 3276 |
| UniProt ID | Q99873 |
| Cytogenetics | 19q13.33 |
| MW | 42.3 kDa |
| Summary | This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. provided by RefSeq, Dec 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001536" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC224239L1 | Lenti ORF clone of Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC224239L2 | Lenti ORF clone of Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC224239L3 | Lenti ORF clone of Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC224239L4 | Lenti ORF clone of Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RG224239 | PRMT1 (tGFP-tagged) - Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1 | 10 ug |
$489.00
$657.00
|
|
| SC309036 | PRMT1 (untagged)-Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1 | 10 ug |
$503.00
|
|
| SC317596 | PRMT1 (untagged)-Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
Discovery of an ApoE4-targeted small-molecule SirT1 enhancer for the treatment of Alzheimer's disease
Campagna, Chandra, Teter et al
Sci Rep (2025) 15 (1), 14028 DOI: 10.1038/s41598-025-96131-2
Campagna, Chandra, Teter et al
Sci Rep (2025) 15 (1), 14028 DOI: 10.1038/s41598-025-96131-2
Cited by 5
AI Summary: In this study, OriGene products—including siRNA kit (#SR418510), siTran 2.0 reagent (#TT320001), pCMV6-Entry vector (#PS100001), and human ORF clones for PRMT1 (#RC224239), PRMT4 (#RC217483), PRMT8 (#RC205188), PRMT5 (#RC203458), and PRMT7 (#RC201672)—were used for gene knockdown and overexpression experiments to investigate the molecular mechanisms underlying SirT1 regulation in Alzheimer's disease models.
Snippet: #SR418510) or 100 nM scrambled siRNA (provided with kit Origene #SR418510) for 48 h using siTran 2.0 reagent following manufacturer's protocol (Origene # TT320001;lls/well were plated in a 24-well plate and cultured at 37 oC with 5% CO2 in DMEM supplemented with FBS and G418 and grown overnight. Cells were then transfected with 0.75 µg of pCMV6-Entry vector (Origene # PS100001) or the human ORF clone for PRMT1 (Origene # RC224239), PRMT4 (Origene # RC217483), PRMT8 (Origene # RC205188), PRMT5 (Origene # RC203458), or PRMT7 (Origene # RC201672) using Turbo
Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy
Aikio, Odeh, Wobst et al
Cell Rep (2025) 44 (1), 115205 DOI: 10.1016/j.celrep.2024.115205
Aikio, Odeh, Wobst et al
Cell Rep (2025) 44 (1), 115205 DOI: 10.1016/j.celrep.2024.115205
Cited by 18
AI Summary: OriGene's PRMT1-Myc_DDK plasmid (Cat #: RC224239) was used in this study to investigate the role of PRMT1-mediated arginine methylation in modulating TDP-43 proteinopathy.
Snippet: RMT1-Myc_DDK Origene Cat #: RC224239 TDP-43M337V-EGFP This Paper N/A
Methylarginine targeting chimeras for lysosomal degradation of intracellular proteins
Seabrook, Franco, Loy et al
Nat Chem Biol (2024)
Seabrook, Franco, Loy et al
Nat Chem Biol (2024)
Cited by 31
AI Summary: OriGene’s PRMT1 plasmid (RC224239) was used to generate the Halo–PRMT1–MYC vector, enabling the study of targeted protein degradation in lysosomes described in this paper.
Snippet: All primers were obtained from IDT. Halo–PRMT1–MYC vector was generated by ligating HaloTag (Addgene, 154144) into the PRMT1 plasmid (OriGene, RC224239) via EcoRI/KpnI;HaloTag primers were as follows: forward 5′-gatcgaattcccaccatggcagaaatcggtactgg-3′ and reverse 5′gatcggtaccggcgtttctcgttcagcttttttgtacaaacttgtacc-3′
PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation
Montenegro, González-Guerrero, Sánchez-Del-Campo et al
Sci Rep (2020) 10 (1), 13275 DOI: 10.1038/s41598-020-70289-3
Montenegro, González-Guerrero, Sánchez-Del-Campo et al
Sci Rep (2020) 10 (1), 13275 DOI: 10.1038/s41598-020-70289-3
Cited by 38
AI Summary: In this study, OriGene products—specifically PRMT1 shRNA (OriGene, #TF311527) and BRCA1 antibody (OriGene, #TA800872)—were used to knock down PRMT1 expression and detect BRCA1 protein, respectively, enabling the authors to investigate how PRMT1-dependent methylation of BRCA1 contributes to breast cancer cell resistance to ionizing radiation.
The Protein Arginine Methyltransferases 1 and 5 affect Myc properties in glioblastoma stem cells
Favia, Salvatori, Nanni et al
Sci Rep (2019) 9 (1), 15925 DOI: 10.1038/s41598-019-52291-6
Favia, Salvatori, Nanni et al
Sci Rep (2019) 9 (1), 15925 DOI: 10.1038/s41598-019-52291-6
AI Summary: OriGene's pCMV6PRMT1-myc-Dkk plasmid was used in this study to express PRMT1 and investigate its role in Myc methylation and function in glioblastoma stem cells.
Snippet: mine 2000 (ThermoFisher Scientific, Waltham, MA, U.S.A.) according to the manufacturer’s instructions. Plasmids were: pCBSFlagMyc33, pCMVPRMT5-6myc (courtesy of dr. Levi A.) and pCMV6PRMT1-myc-Dkk (Origene, Rockville, MD, U.S.A), together with the corresponding empty vectors. The shMyc insert was subcloned from the KRAB lentiviral vector into the Tet-on pSLIK lentivirus33. Lentiviral production
Hepatocyte PRMT1 protects from alcohol induced liver injury by modulating oxidative stress responses
Zhao, Adams, Weinman et al
Sci Rep (2019) 9 (1), 9111 DOI: 10.1038/s41598-019-45585-2
Zhao, Adams, Weinman et al
Sci Rep (2019) 9 (1), 9111 DOI: 10.1038/s41598-019-45585-2
AI Summary: OriGene’s pCMV6-PRMT1 vector was used in this study to express and analyze PRMT1 and its mutants, enabling the authors to investigate the role of PRMT1 in protecting hepatocytes from alcohol-induced liver injury.
Snippet: n and 50 mg mL−1 streptomycin. Cells were transfected using Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer’s protocol. Vectors pCMV6-PRMT1 vector was from Origene. The PRMT1 point mutations were generated by site directed mutagenesis (Quickchange kit, Stratagene). AAV8.TBG.PI.Null, AAV8.TBG.PI.Cre, AAV8-U6-JMJD6shRNA and AAV8-U6-ScrambledshRNA were from
Showing 1-6 of 11 papers.
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