PHF19 (NM_015651) Human Tagged ORF Clone

SKU RC221894

PHF19 (Myc-DDK-tagged)-Human PHD finger protein 19 (PHF19), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PHF19
• Synonyms: MTF2L1; PCL3; TDRD19B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221894 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGAATCGAGCTCTGGATCCAGGGACTCGGGACTCCTATGGTGCCACCAGCCACCTCCCCAACAAGG; GGGCCCTGGCGAAGGTCAAGAACAACTTCAAAGACTTGATGTCCAAACTGACGGAGGGCCAGTATGTGCT; GTGCCGGTGGACAGATGGCCTGTACTACCTCGGGAAGATCAAGAGGGTCAGCAGCTCTAAGCAAAGCTGC; CTCGTGACTTTCGAAGATAATTCCAAATACTGGGTCCTATGGAAGGACATACAGCATGCCGGTGTTCCAG; GAGAGGAGCCCAAGTGCAACATCTGCCTAGGGAAGACATCAGGGCCGCTGAATGAGATCCTCATCTGCGG; GAAGTGTGGCCTGGGTTACCACCAGCAGTGCCACATCCCCATAGCGGGCAGTGCTGACCAGCCCCTGCTC; ACACCTTGGTTCTGCCGACGCTGCATCTTCGCACTGGCTGTGCGGAAAGGCGGCGCGCTGAAGAAGGGCG; CCATCGCCAGGACGCTGCAGGCCGTGAAGATGGTGCTGTCCTACCAGCCCGAGGAGCTCGAGTGGGACTC; GCCCCATCGCACCAACCAGCAGCAATGCTACTGCTACTGCGGCGGGCCCGGAGAATGGTACCTGCGGATG; CTGCAATGTTACCGGTGCAGGCAGTGGTTCCACGAGGCCTGCACCCAGTGCCTCAATGAGCCCATGATGT; TTGGAGACCGGTTTTACCTGTTCTTCTGCTCCGTGTGTAACCAGGGCCCAGAGTACATCGAGAGGCTGCC; CCTGCGATGGGTGGATGTGGTTCACCTGGCCCTCTATAATCTGGGGGTACAGAGCAAGAAGAAGTACTTT; GACTTTGAGGAGATTCTGGCCTTTGTCAACCACCACTGGGAGCTCCTGCAGCTTGGCAAGCTCACCAGCA; CCCCAGTGACAGATCGAGGACCACATCTCCTCAACGCTCTGAACAGTTATAAAAGCCGGTTCCTCTGCGG; CAAGGAGATCAAGAAGAAGAAGTGCATCTTCCGCCTGCGCATCCGCGTCCCACCCAACCCGCCAGGGAAG; CTGCTGCCTGACAAAGGACTGCTGCCAAATGAGAACAGCGCCTCCTCTGAGCTGCGTAAGAGAGGAAAGA; GCAAGCCTGGTTTGTTGCCTCACGAATTCCAGCAGCAGAAAAGGCGAGTTTATAGAAGAAAAAGATCAAA; GTTTTTGCTGGAAGATGCTATTCCCAGTAGTGACTTCACCTCAGCCTGGAGCACCAACCACCACCTGGCT; AGCATATTTGACTTCACGCTGGATGAAATTCAAAGTTTAAAAAGTGCCAGCTCAGGCCAGACCTTCTTCT; CAGATGTCGACTCCACCGACGCTGCCAGCACCTCTGGCTCTGCCTCCACCAGCCTCTCCTATGACTCCAG; ATGGACAGTGGGCAGCCGAAAGAGGAAGCTGGCAGCCAAGGCATACATGCCCCTGCGGGCAAAGCGGTGG; GCAGCTGAGCTGGATGGACGCTGCCCCTCGGACAGCAGTGCAGAGGGGGCTTCAGTCCCCGAGCGGCCAG; ACGAAGGCATTGACAGCCACACATTTGAGAGCATCAGTGAAGATGACTCATCCCTGTCCCACCTCAAGTC; ATCTATCACCAACTACTTTGGTGCAGCTGGGCGGTTGGCCTGTGGGGAGAAGTACCAGGTGTTGGCTCGG; AGGGTCACACCTGAGGGCAAGGTTCAGTACCTGGTGGAGTGGGAAGGGACCACCCCTTAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221894 protein sequence; Red=Cloning site Green=Tags(s); ; MENRALDPGTRDSYGATSHLPNKGALAKVKNNFKDLMSKLTEGQYVLCRWTDGLYYLGKIKRVSSSKQSC; LVTFEDNSKYWVLWKDIQHAGVPGEEPKCNICLGKTSGPLNEILICGKCGLGYHQQCHIPIAGSADQPLL; TPWFCRRCIFALAVRKGGALKKGAIARTLQAVKMVLSYQPEELEWDSPHRTNQQQCYCYCGGPGEWYLRM; LQCYRCRQWFHEACTQCLNEPMMFGDRFYLFFCSVCNQGPEYIERLPLRWVDVVHLALYNLGVQSKKKYF; DFEEILAFVNHHWELLQLGKLTSTPVTDRGPHLLNALNSYKSRFLCGKEIKKKKCIFRLRIRVPPNPPGK; LLPDKGLLPNENSASSELRKRGKSKPGLLPHEFQQQKRRVYRRKRSKFLLEDAIPSSDFTSAWSTNHHLA; SIFDFTLDEIQSLKSASSGQTFFSDVDSTDAASTSGSASTSLSYDSRWTVGSRKRKLAAKAYMPLRAKRW; AAELDGRCPSDSSAEGASVPERPDEGIDSHTFESISEDDSSLSHLKSSITNYFGAAGRLACGEKYQVLAR; RVTPEGKVQYLVEWEGTTPY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015651
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015651.3
• RefSeq Size: 4310 bp
• RefSeq ORF: 1743 bp
• Locus ID: 26147
• UniProt ID: Q5T6S3
• Cytogenetics: 9q33.2
• Protein Families: Druggable Genome
• MW: 65.6 kDa
• Summary: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.[UniProtKB/Swiss-Prot Function]