PHF19 (NM_015651) Human Untagged Clone

SKU SC304338

PHF19 (untagged)-Human PHD finger protein 19 (PHF19), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PHF19
• Synonyms: MTF2L1; PCL3; TDRD19B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304338 representing NM_015651.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGAATCGAGCTCTGGATCCAGGGACTCGGGACTCCTATGGTGCCACCAGCCACCTCCCCAACAAG; GGGGCCCTGGCGAAGGTCAAGAACAACTTCAAAGACTTGATGTCCAAACTGACGGAGGGCCAGTATGTG; CTGTGCCGGTGGACAGATGGCCTGTACTACCTCGGGAAGATCAAGAGGGTCAGCAGCTCTAAGCAAAGC; TGCCTCGTGACTTTCGAAGATAATTCCAAATACTGGGTCCTATGGAAGGACATACAGCATGCCGGTGTT; CCAGGAGAGGAGCCCAAGTGCAACATCTGCCTAGGGAAGACATCAGGGCCGCTGAATGAGATCCTCATC; TGCGGGAAGTGTGGCCTGGGTTACCACCAGCAGTGCCACATCCCCATAGCGGGCAGTGCTGACCAGCCC; CTGCTCACACCTTGGTTCTGCCGACGCTGCATCTTCGCACTGGCTGTGCGGAAAGGCGGCGCGCTGAAG; AAGGGCGCCATCGCCAGGACGCTGCAGGCCGTGAAGATGGTGCTGTCCTACCAGCCCGAGGAGCTCGAG; TGGGACTCGCCCCATCGCACCAACCAGCAGCAATGCTACTGCTACTGCGGCGGGCCCGGAGAATGGTAC; CTGCGGATGCTGCAATGTTACCGGTGCAGGCAGTGGTTCCACGAGGCCTGCACCCAGTGCCTCAATGAG; CCCATGATGTTTGGAGACCGGTTTTACCTGTTCTTCTGCTCCGTGTGTAACCAGGGCCCAGAGTACATC; GAGAGGCTGCCCCTGCGATGGGTGGATGTGGTTCACCTGGCCCTCTATAATCTGGGGGTACAGAGCAAG; AAGAAGTACTTTGACTTTGAGGAGATTCTGGCCTTTGTCAACCACCACTGGGAGCTCCTGCAGCTTGGC; AAGCTCACCAGCACCCCAGTGACAGATCGAGGACCACATCTCCTCAACGCTCTGAACAGTTATAAAAGC; CGGTTCCTCTGCGGCAAGGAGATCAAGAAGAAGAAGTGCATCTTCCGCCTGCGCATCCGCGTCCCACCC; AACCCGCCAGGGAAGCTGCTGCCTGACAAAGGACTGCTGCCAAATGAGAACAGCGCCTCCTCTGAGCTG; CGTAAGAGAGGAAAGAGCAAGCCTGGTTTGTTGCCTCACGAATTCCAGCAGCAGAAAAGGCGAGTTTAT; AGAAGAAAAAGATCAAAGTTTTTGCTGGAAGATGCTATTCCCAGTAGTGACTTCACCTCAGCCTGGAGC; ACCAACCACCACCTGGCTAGCATATTTGACTTCACGCTGGATGAAATTCAAAGTTTAAAAAGTGCCAGC; TCAGGCCAGACCTTCTTCTCAGATGTCGACTCCACCGACGCTGCCAGCACCTCTGGCTCTGCCTCCACC; AGCCTCTCCTATGACTCCAGATGGACAGTGGGCAGCCGAAAGAGGAAGCTGGCAGCCAAGGCATACATG; CCCCTGCGGGCAAAGCGGTGGGCAGCTGAGCTGGATGGACGCTGCCCCTCGGACAGCAGTGCAGAGGGG; GCTTCAGTCCCCGAGCGGCCAGACGAAGGCATTGACAGCCACACATTTGAGAGCATCAGTGAAGATGAC; TCATCCCTGTCCCACCTCAAGTCATCTATCACCAACTACTTTGGTGCAGCTGGGCGGTTGGCCTGTGGG; GAGAAGTACCAGGTGTTGGCTCGGAGGGTCACACCTGAGGGCAAGGTTCAGTACCTGGTGGAGTGGGAA; GGGACCACCCCTTACTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_015651
• Insert Size: 1743 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015651.2
• RefSeq Size: 4310 bp
• RefSeq ORF: 1743 bp
• Locus ID: 26147
• UniProt ID: Q5T6S3
• Cytogenetics: 9q33.2
• Protein Families: Druggable Genome
• MW: 65.6 kDa
• Summary: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) uses an alternate 5' terminal exon and it thus differs in its 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 3. The encoded isoform (a, also known as hPCL3L) is shorter at the N-terminus, compared to isoform c.