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GRAMD2A (NM_001012642) Human Tagged ORF Clone

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SKU
RC220145
GRAMD2 (Myc-DDK-tagged)-Human GRAM domain containing 2 (GRAMD2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GRAMD2A
Synonyms GRAMD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220145 representing NM_001012642
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGCTTTAAGCCGGAGCGAGGCCACCGAGGAGGGCGGCAACCAACAAATGCACAGAAAGACAGCTT
CTCTGAACAGTCCTGTGTCCTGCAAAGAGAAACCAGACAGAGTTGAGGAGCCCCCGGACTACAGTCTGCA
CTGGCCAGAAGGCTTGAAGGGTGAAGAGATAAAGAAGTGTGGCCGAGAAGGGATAACACTGAATAAATAC
AACCAGCAATACCACAAGCTGTTTAAGGATGTTCCCTTGGAGGAAGTGGTTCTCAAAGTGTGTTCCTGTG
CCCTCCAGAGGGACTTCCTCCTCCAGGGCCGGCTCTACATCTCCCCCAACTGGCTCTGCTTCCATGCCAG
CCTCTTTGGCAAGGATATCAAGGTGGTCATTCCTGTGGTGTCTGTGCAAATGATCAAAAAACACAAGATG
GCACGGCTCCTTCCCAATGGACTGGCCATCACCACCAACACCAGCCAGAAGTATATCTTTGTGTCACTGC
TCTCCCGGGACAGTGTATATGACCTGCTGAGGAGAGTCTGCACCCACCTACAGCCTTCCAGCAAGAAGAG
TCTGAGTGTAAGAGAATTTTCAGGGGAACCTGAGTCTCTGGAAGTCCTCATCCCTGAGATGAAGTGGAGA
AAGGTATGCCCTTCCTCCAGGTCCCTGTCTCTCCCAGACAACATCCCTTGTATCCCTCCATCATCCGTGG
ACTCCACAGACAGTTTCTTCCCCTCCAGGAAGCCTCCAATGTCTGAAAAGTCAAGAGCCCAAGTAGCTTC
AGAAAATGGTGGGAGGTGGGCATGGCCCATGCCAGGCTGGGGTCCTGCCTGCCCTAAGAAGATGCCGAAC
TGCTCTCCCACTGCAAAGAATGCTGTCTATGAGGAGGATGAGCTGGAGGAGGAGCCCAGGAGCACTGGGG
AGCTGAGGCTCTGGGATTACCGGCTCCTCAAGGTCTTCTTTGTGCTGATCTGCTTCCTGGTCATGTCCTC
ATCCTACCTGGCGTTCCGTATTTCTCGGCTAGAGCAGCAGTTATGCTCCTTGAGTTGGGATGACCCAGTC
CCTGGGCACAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220145 representing NM_001012642
Red=Cloning site Green=Tags(s)

MTALSRSEATEEGGNQQMHRKTASLNSPVSCKEKPDRVEEPPDYSLHWPEGLKGEEIKKCGREGITLNKY
NQQYHKLFKDVPLEEVVLKVCSCALQRDFLLQGRLYISPNWLCFHASLFGKDIKVVIPVVSVQMIKKHKM
ARLLPNGLAITTNTSQKYIFVSLLSRDSVYDLLRRVCTHLQPSSKKSLSVREFSGEPESLEVLIPEMKWR
KVCPSSRSLSLPDNIPCIPPSSVDSTDSFFPSRKPPMSEKSRAQVASENGGRWAWPMPGWGPACPKKMPN
CSPTAKNAVYEEDELEEEPRSTGELRLWDYRLLKVFFVLICFLVMSSSYLAFRISRLEQQLCSLSWDDPV
PGHR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001012642
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001012642.3
RefSeq Size 3280 bp
RefSeq ORF 1065 bp
Locus ID 196996
UniProt ID Q8IUY3
Cytogenetics 15q23
Protein Families Transmembrane
MW 40.1 kDa
Summary Participates in the organization of endoplasmic reticulum-plasma membrane contact sites (EPCS) with pleiotropic functions including STIM1 recruitment and calcium homeostasis. Constitutive tether that co-localize with ESYT2/3 tethers at endoplasmic reticulum-plasma membrane contact sites in a phosphatidylinositol lipid-dependent manner. Pre-marks the subset of phosphtidylinositol 4,5-biphosphate (PI(4,5)P2)-enriched EPCS destined for the store operated calcium entry pathway (SOCE).UniProtKB/Swiss-Prot Function
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"NM_001012642" in other vectors (4)
SKU Description Size Price
RC220145L3 Lenti ORF clone of Human GRAM domain containing 2 (GRAMD2), Myc-DDK-tagged 10 ug
$757.00
RC220145L4 Lenti ORF clone of Human GRAM domain containing 2 (GRAMD2), mGFP tagged 10 ug
$757.00
RG220145 GRAMD2 (tGFP-tagged) - Human GRAM domain containing 2 (GRAMD2) 10 ug
$657.00
SC301686 GRAMD2 (untagged)-Human GRAM domain containing 2 (GRAMD2) 10 ug
$503.00

Plasmid Map for RC220145

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220145
Circular map for RC220145

file-alt Citations

GRAM domain proteins specialize functionally distinct ER-PM contact sites in human cells
Besprozvannaya, Dickson, Li et al
Elife (2018) 7 DOI: 10.7554/eLife.31019
AI Summary: OriGene cDNA clones for human GRAMD1a (RC226692) and GRAMD2a (RC220145) were used to express these proteins in human cells, enabling the study of their distinct roles at ER-plasma membrane contact sites.
Snippet: …cDNA clones of human GRAMD1a and GRAMD2a/GRAMD2 were obtained from Origene (GRAMD1a: RC226692, GRAMD2a: RC220145) and cloned into pAcGFP1-N1 and pAcmCherry-N1 vectors. …cDNA clones of human GRAMD1a and GRAMD2a/GRAMD2 were obtained from Origene (GRAMD1a: RC226692, GRAMD2a: RC220145) and cloned into pAcGFP1-N1 and pAcmCherry-N1 vectors. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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